Anti-Raf1 (phospho S621)抗体
参阅全部 Raf1 一抗
兔多克隆抗体to Raf1 (phospho S621)
Rabbit
适用于: WBmore details
与反应: Human
预测可用于: Mouse, Rat
Synthetic peptide corresponding to Human Raf1 (phospho S621).
Database link: P04049
Immunoprecipitates or cell lysates from EGF-stimulated Hek293 cells transfected with c-Raf.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free
浓度
50 µl 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated c-Raf. The final product is generated by affinity chromatography using a c-Raf-derived peptide that is phosphorylated at serine 621.
多克隆
IgG
Abpromise™承诺保证使用ab4767于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 74 kDa. |
Entrez Gene: 5894 Human
Entrez Gene: 110157 Mouse
Omim: 164760 Human
SwissProt: P04049 Human
SwissProt: Q99N57 Mouse
Unigene: 159130 Human
Unigene: 184163 Mouse
Unigene: 33262 Rat
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Western blot - Anti-Raf1 (phospho S621) antibody (ab4767)
Peptide Competition and Mutant Analysis:
Immunoprecipitates prepared from Hek293 cells stimulated with EGF and overexpressing wild-type c-Raf (1-4) or c-Raf mutant S621A (5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4767 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: the phosphopeptide immunogen (1), a generic phosphoserine containing peptide (2), the non-phosphopeptide corresponding to the immunogen (3), or, no peptide (4, 5). The smaller blot presented below shows the relative amount of protein in the wild-type vs. the S621A mutant protein via a c-Raf pan antibody. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide c
