Anti-TRIM2抗体
山羊多克隆抗体to TRIM2
Goat
适用于: IHC-P, WBmore details
与反应: Rat, Human
预测可用于: Cow, Dog
Synthetic peptide corresponding to Human TRIM2 aa 731-744 (C terminal).
Sequence:
DSGNHCFKVYRYLQ
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab3942于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | Use a concentration of 6 - 12 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
WB | Use a concentration of 0.5 µg/ml. A 1 hour primary incubation is recommended for this product. |
Entrez Gene: 23321 Human
Omim: 614141 Human
SwissProt: Q9C040 Human
Unigene: 435711 Human
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Western blot - Anti-TRIM2 antibody (ab3942)
Anti-TRIM2 antibody (ab3942) at 0.5 µg/ml + Rat brain tissue lysate at 35 µg
Observed band size: 80,85 kDawhy is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was for 1 hour. Detected by chemiluminescence.