Anti-Paxillin (phospho Y31)抗体
参阅全部 Paxillin 一抗
兔多克隆抗体to Paxillin (phospho Y31)
Rabbit
适用于: ICC/IF, WBmore details
与反应: Mouse, Human
预测可用于: Chicken
Synthetic peptide corresponding to Human Paxillin (phospho Y31).
ICC: NIH/3T3 (mouse embryo fibroblast cell line), NMµMG (mouse mammary) cells. WB: A431 (human epidermoid carcinoma cell line), HeLa (human epithelial cell line from cervix adenocarcinoma), NIH/3T3 (mouse embryo fibroblast cell line), A549 (human lung carcinoma cell line) cell lysate.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
浓度
50 µl 浓度为 0.3 mg/ml
Immunogen affinity purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin, and ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at tyrosine 31.
多克隆
IgG
Abpromise™承诺保证使用ab4832于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/250. | |
| WB | 1/1000. Predicted molecular weight: 68 kDa. |
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Unigene: 18714 Mouse
Entrez Gene: 395832 Chicken
Entrez Gene: 5829 Human
Entrez Gene: 19303 Mouse
Omim: 602505 Human
SwissProt: P49024 Chicken
SwissProt: P49023 Human
SwissProt: Q8VI36 Mouse
Unigene: 446336 Human
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Immunocytochemistry/ Immunofluorescence - Anti-Paxillin (phospho Y31) antibody (ab4832)
Immunocytochemistry/ Immunofluorescence of NIH/3T3 (mouse embryo fibroblast cell line) treated with 50 ng of PDGF for 10 minutes with ab4832 (1/250). A Goat anti-Rabbit IgG (H+L) Alexa Fluor® 488 conjugate (1/2000) was used as a secondary (Green). DAPI (Blue) was used as a counter stain. F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300). Panel e is untreaded cells and panel f had no primary antibody.
Western blot - Anti-Paxillin (phospho Y31) antibody (ab4832)
All lanes : Anti-Paxillin (phospho Y31) antibody (ab4832)
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate treated for 1 Hr with 100 µM of Pervanadate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated for 20 minutes with 50 ng/mL of TNF alpha
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 6 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate treated for 10 minutes with 50 ng/mL of PDGF
Lane 7 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 8 : A549 (human lung carcinoma cell line) whole cell lysate treated for 20 minutes with 200 nM of PMA
Lysates/proteins at 20 µl per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 60 kDawhy is the actual band size different from the predicted?
ab4832 was diluted between 1/500 and 1/2000.
Immunocytochemistry/ Immunofluorescence - Anti-Paxillin (phospho Y31) antibody (ab4832)
Colocalization of phospho Paxillin and actin in TGF-beta treated NMµMG (mouse mammary) cells. Green = actin stress fibers, Orange = Paxillin [pY31], Yellow = colocalization.
