Anti-Glucocorticoid Receptor抗体
参阅全部 Glucocorticoid Receptor 一抗
兔多克隆抗体to Glucocorticoid Receptor
Rabbit
This antibody detects both the unactivated and activated forms of GR.
适用于: IHC-P, ICC/IF, WBmore details
与反应: Mouse, Human, Snake
Synthetic peptide corresponding to Human Glucocorticoid Receptor aa 150-175.
Sequence:
APTEKEFPKTHSDVSSEQQHLKGQTG
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab3671于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/250. | |
| ICC/IF | (1) | 1/250. |
| WB | (2) | 1/500 - 1/2500. Detects a band of approximately 97 kDa (predicted molecular weight: 86 kDa). |
Entrez Gene: 2908 Human
Entrez Gene: 14815 Mouse
Omim: 138040 Human
SwissProt: P04150 Human
SwissProt: P06537 Mouse
Unigene: 122926 Human
Unigene: 129481 Mouse
GCRST antibody
glucocorticoid nuclear receptor variant 1 antibody
Glucocorticoid receptor antibody
GR antibody
GRL antibody
Grl1 antibody
nr3c1 antibody
Nuclear receptor subfamily 3 group C member 1 antibody
nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
GCCR antibody
GCR antibody
GCR_HUMAN antibody
Western blot - Anti-Glucocorticoid Receptor antibody (ab3671)
All lanes : Anti-Glucocorticoid Receptor antibody (ab3671) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) membrane enriched extract
Lane 2 : MCF7 (human breast adenocarcinoma cell line) membrane enriched extract
Lane 3 : T-47D membrane enriched extract
Lane 4 : MDA-MB-231 (human breast adenocarcinoma cell line) membrane enriched extract
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) membrane enriched extract
Lane 6 : Mouse brain tissue extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 86 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3671)
Immunocytochemistry/Immunofluorescence analysis of HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3671)
Immunocytochemistry/Immunofluorescence analysis of A2058 (Human metastatic melanoma cell line) cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3671)
Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial adenocarcinoma cell line) cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3671)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Thamnophis sirtalis (Common garter snake) brain tissue sections labeling Glucocorticoid Receptor with ab3671 at 1/250. Samples were blocked with 10% goat serum in 0.1M PBS. Samples were incubated with the primary antibody for 48 hours at 4°C. A biotin-conjugated goat anti-rabbit was used as the secondary antibody.
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