Anti-SERCA2 ATPase抗体
参阅全部 SERCA2 ATPase 一抗
兔多克隆抗体to SERCA2 ATPase
Rabbit
适用于: IP, WB, ICC/IF, IHC-Pmore details
与反应: Mouse, Rat, Rabbit, Human, Pig
预测可用于: Chicken, Cat, Dog
Synthetic peptide within Human SERCA2 ATPase aa 250-550. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_733765.1 (GeneID 488).
Database link: P16615
WB: HeLa, HEK-293T, NIH/3T3 whole cell lysate. Pig and rabbit aorta homogenate. Rat aortic smooth muscle cell lysate. IHC-P: Human heart tissue. IP: SERCA2 ATPase IP in HEK-293T whole cell lysate. ICC/IF: HeLa cells.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3625于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | (1) | Use a concentration of 1 - 4 µg/ml. |
| WB | (3) | 1/1000 - 1/5000. Predicted molecular weight: 115 kDa. |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IHC-P | (2) | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Entrez Gene: 396446 Chicken
Entrez Gene: 488 Human
Entrez Gene: 11938 Mouse
Entrez Gene: 100038308 Rabbit
Omim: 108740 Human
SwissProt: Q03669 Chicken
SwissProt: P16615 Human
SwissProt: O55143 Mouse
SwissProt: P20647 Rabbit
Unigene: 506759 Human
Unigene: 227583 Mouse
Unigene: 2305 Rat
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Western blot - Anti-SERCA2 ATPase antibody (ab3625)
All lanes : Anti-SERCA2 ATPase antibody (ab3625) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 115 kDa
Exposure time: 30 seconds
Western blot - Anti-SERCA2 ATPase antibody (ab3625)
Sample: Homogenate from Pig (lanes 1 and 2) or Rabbit (lane 4) aorta or lysate from cultured Rat aortic smooth muscle cells (lane 3)
Affinity purified Rabbit anti-SERCA2 (ab3625) (lanes 2, 3 and IP for lane 4) or control (ctrl) monoclonal anti-SERCA2 (lanes 1 and WB for lane 4).
Dilutions: 1:1,000 (lanes 1, 2 and 4) or 1:2,500 (lane 3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody (ab3625)
Ab3625 staining human heart. Staining is localised to the cytoplasm/sarcoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Immunoprecipitation - Anti-SERCA2 ATPase antibody (ab3625)
SERCA2 ATPase was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab3625 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab3625 at 1 µg/ml.
Lane 1: ab3625 IP in HEK-293T whole cell lysate.
Lane 3: ab3625 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody (ab3625)
ICC/IF image of ab3625 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3625, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
