Anti-Tau (phospho T205)抗体
参阅全部 Tau 一抗
兔多克隆抗体to Tau (phospho T205)
Rabbit
The specificity of this antibody refers to P10636-8.
适用于: IHC-Fr, Flow Cyt, WBmore details
与反应: Mouse, Human
预测可用于: Rat
Synthetic peptide corresponding to Human Tau (phospho T205).
Database link: P10636-8
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Immunogen affinity purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated tau. The final product is generated by affinity chromatography using a tau-derived peptide that is phosphorylated at threonine 205.
多克隆
IgG
Abpromise™承诺保证使用ab4841于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | ||
| Flow Cyt | ||
| WB |
应用说明
IHC-Fr: Use at an assay dependent dilution.
WB: 1/1000. Predicted molecular weight: 62 kDa. Can be blocked with Tau peptide - phospho T205 (phospho and non-phospho pair).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Entrez Gene: 4137 Human
Entrez Gene: 17762 Mouse
Omim: 157140 Human
SwissProt: P10636 Human
SwissProt: P10637 Mouse
Unigene: 101174 Human
Unigene: 1287 Mouse
Unigene: 2455 Rat
There are 9 isoforms produced by alternative splicing.
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Western blot - Anti-Tau (phospho T205) antibody (ab4841)
WB using Ab4841. The peptide corresponding to tau [pT205] blocks the antibody signal, thereby demonstrating the specificity of the antibody.
Flow Cytometry - Anti-Tau (phospho T205) antibody (ab4841)
Flow cytometric analysis of 70% ethanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cell line labeling Tau (phospho T205) with ab4841 at 5 μg/million cells (red) compared with a rabbit Isotype control details (pink),an unlabelled control (purple) and a secondary only control (green). Alexa Fluor® 488 Goat Anti-Rabbit, at 1/400 dilution was used as the secondary antibody.
