Anti-IgA Secretory Component抗体[SC-05]
参阅全部 IgA Secretory Component 一抗
小鼠单克隆抗体[SC-05] to IgA Secretory Component
Mouse
ab3924 reacts with 80 kDa human secretory component glycoprotein (both free and bound in secretory IgA). The recognized glycoprotein is a specific membrane marker of glandular carcinomas.
适用于: WBmore details
与反应: Human
Full length native protein (purified) corresponding to Human IgA Secretory Component. Affinity-purified secretory component from human colostrum.
WB: MCF7 and MDA-MB-361 cell lysates.
Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS
浓度
100 µg 浓度为 1 mg/ml
Protein A purified
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.
Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.
单克隆
SC-05
IgG1
Protein A purified
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.
Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.
单克隆
SC-05
IgG1
Abpromise™承诺保证使用ab3924于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa). See Abreview. Use under non-reducing conditions. |
![Western blot - Anti-IgA Secretory Component antibody [SC-05] (ab3924)](https://www.abcam.cn/ps/products/3/ab3924/Images/ab3924-8412-anti-iga-secretory-component-antibody-sc-05-western-blot.jpg)
Western blot - Anti-IgA Secretory Component antibody [SC-05] (ab3924)
All lanes : Anti-IgA Secretory Component antibody [SC-05] (ab3924) at 1 µg/ml
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 68 kDawhy is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Secretory Component Glycoprotein contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
