Anti-Paxillin (phospho Y118) antibody

• 产品名称

  Anti-Paxillin (phospho Y118)抗体
  参阅全部 Paxillin 一抗

• 描述

  兔多克隆抗体to Paxillin (phospho Y118)

• 宿主

  Rabbit

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Mouse, Human, African green monkey
  预测可用于: Chicken

• 免疫原

  Synthetic peptide corresponding to Human Paxillin (phospho Y118).

• 阳性对照

• WB: COS-7, A431, HeLa whole cell lysates

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.30
  Preservative: 0.05% Sodium azide
  Constituents: PBS, 0.1% BSA
  
  BSA is IgG and protease free

• 浓度

• 批次浓度范围 50 µl 浓度为 0.5 - 0.8 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin, and ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at tyrosine 31.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab4833于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 395832 Chicken

• Entrez Gene: 5829 Human

• Entrez Gene: 19303 Mouse

• Omim: 602505 Human

• SwissProt: P49024 Chicken

• SwissProt: P49023 Human

• SwissProt: Q8VI36 Mouse

• Unigene: 446336 Human

• Unigene: 18714 Mouse

别名

• FLJ16691 antibody

• FLJ23042 antibody

• Paired box protein Pax 1 antibody

• PAX 1 antibody

• PAX1 antibody

• PAXI_HUMAN antibody

• Paxillin alpha antibody

• Paxillin antibody

• PXN antibody

• PXN protein antibody

• 

  Western blot - Anti-Paxillin (phospho Y118) antibody (ab4833)

  All lanes : Anti-Paxillin (phospho Y118) antibody (ab4833) at 1/500 dilution
  
  Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
  Lane 2 : COS-7 whole cell lysate treated with 100 uM Pervanadate for 1 hour
  Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
  Lane 4 : A431 whole cell lysate treated with 100 uM Pervanadate for 1 hour
  Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
  Lane 6 : HeLa whole cell lysate treated with 100 uM Pervanadate for 1 hour
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/5000 dilution
  
  Predicted band size: 68 kDa
  
  
  
  

  A 66 kDa band corresponding to Paxillin pTyr118 was enhanced across treated cell lines. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.

• 

  Western blot - Anti-Paxillin (phospho Y118) antibody (ab4833)

  All lanes : Anti-Paxillin (phospho Y118) antibody (ab4833) at 0.75 µg/ml (3% BSA-TBST buffer)
  
  All lanes : NMµMG (mouse mammary) cells transfected with EGFP-tagged paxillin
  
  Secondary
  All lanes : goat F(ab’)2 anti-rabbit IgG alkaline phosphatase
  
  Predicted band size: 68 kDa
  
  
  
  

  Peptide Competition: Extracts were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC.

  Prior incubation with:

  1- no peptide,

  2- the non-phosphopeptide corresponding to the immunogen

  3- a generic phosphotyrosine containing peptide

  4- the phosphopeptide immunogen 

  Bands were detected using the Tropix WesternStar method.

  The data show that only the peptide corresponding to ab4833 blocks the antibody signal, thereby demonstrating the specificity of the antibody.