Anti-Rad17 (phospho S645)抗体
参阅全部 Rad17 一抗
兔多克隆抗体to Rad17 (phospho S645)
Rabbit
适用于: IHC-P, WBmore details
与反应: Mouse, Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, African green monkey, Orangutan
Synthetic peptide corresponding to Human Rad17 (phospho S645). Phosphorylated synthetic peptide, which represented a portion of human Rad17 (GeneID 5884) around serine 645 according to the numbering given in entry NP_002864.1.
IHC: human colon carcinoma tissue; mouse renal cell carcinoma tissue; WB: HeLa and HEK293T treated with Etoposide
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µl 浓度为 1 mg/ml
Immunogen affinity purified
Antibodies that were not phospho-specific were removed by solid phase absorption. Antibodies specific for Rad 17pSer 645 were affinity purified using the phospho peptide immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab3620于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/5000. Detects a band of approximately 85-88 kDa (predicted molecular weight: 77 kDa). |
Entrez Gene: 5884 Human
Entrez Gene: 19356 Mouse
Omim: 603139 Human
SwissProt: O75943 Human
SwissProt: Q6NXW6 Mouse
Unigene: 16184 Human
Unigene: 248489 Mouse
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Western blot - Anti-Rad17 (phospho S645) antibody (ab3620)
All lanes : Anti-Rad17 (phospho S645) antibody (ab3620) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate treated with Etoposide
Lane 2 : HeLa whole cell lysate mock-treated
Lane 3 : HEK293T whole cell lysate treated with Etoposide
Lane 4 : HEK293T whole cell lysate mock-treated
Lysates/proteins at 50 µg per lane.
Predicted band size: 77 kDa
Exposure time: 30 seconds
Lower panel: membrane was reblotted with goat anti-Rad17 antibody to examine total Rad17.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad17 (phospho S645) antibody (ab3620)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling Rad17 with ab3620 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad17 (phospho S645) antibody (ab3620)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling Rad17 with ab3620 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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