Anti-UBE2C antibody

• 产品名称

  Anti-UBE2C抗体
  参阅全部 UBE2C 一抗

• 描述

  山羊多克隆抗体to UBE2C

• 宿主

  Goat

• 经测试应用

  适用于: WB, IPmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide: QETYSKQVTSQEP, corresponding to C terminal amino acids 167-179 of Human UBE2C.

  Run BLAST with  Run BLAST with 

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 0.5% Tris, 0.5% BSA

• 浓度

• 100 µg 浓度为 0.5 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab3935于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 11065 Human

• Omim: 605574 Human

• SwissProt: O00762 Human

• Unigene: 93002 Human

• 别名

• UB E2C antibody

• UBCH 10 antibody

• UbcH10 antibody

• UBE 2C antibody

• Ube2c antibody

• UBE2C_HUMAN antibody

• Ubiquitin carrier protein C antibody

• Ubiquitin carrier protein E2 C antibody

• Ubiquitin carrier protein E2C antibody

• Ubiquitin conjugating enzyme E2 C antibody

• Ubiquitin conjugating enzyme E2C antibody

• Ubiquitin protein ligase C antibody

• Ubiquitin-conjugating enzyme E2 C antibody

• Ubiquitin-protein ligase C antibody

• Cyclin selective ubiquitin carrier protein antibody

• dJ447F3.2 antibody

• Mitotic specific ubiquitin conjugating enzyme antibody

• 

  Western blot - Anti-UBE2C antibody (ab3935)

  ab3935 at a 2.5µg/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL):

  Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2µl).
  Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2µl).
  Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20µl).
  Lane 4 = Asynchronous HeLa cell whole cell lysate (20µl).
  
  For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.

  ab3935 at a 2.5µg/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL):
  
  Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2µl).
  Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2µl).
  Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20µl).
  Lane 4 = Asynchronous HeLa cell whole cell lysate (20µl).
  
  For a more detailed descri

• 

  Immunoprecipitation - Anti-UBE2C antibody (ab3935)

  ab3935 staining approximately 20 kDa human UBE2 after immmunoprecipitation from HeLa whole cell lysate (106 cells/ml) using 40µl of ab3935 saturated protein G dynabeads, by Western blot (ECL):

  Lane 1 = IP from Noc arrested HeLa cell extract (beads only).
  Lane 2 = IP from Noc arrested HeLa cell extract (goat whole serum coupled to beads).
  Lane 3 = IP from Noc arrested HeLa cell extract (ab3935 coupled to beads)
  Lane 4 = Noc arrested HeLa whole cell lysate.

  For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.