Anti-MLH3抗体
参阅全部 MLH3 一抗
山羊多克隆抗体to MLH3
Goat
适用于: ICC/IFmore details
与反应: Human
Synthetic peptide:
RQSLQQSMPPCEPP
, corresponding to C terminal amino acids 1416-1429 of Human MLH3.
Gene Ontology terms - satellite DNA binding; ATP binding; molecular_function unknown; mismatch repair; biological_process unknown; nucleus; cellular_component unknown
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab4834于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | Use a concentration of 10 µg/ml. |
Entrez Gene: 27030 Human
GenBank: NP_055196 Human
Omim: 604395 Human
SwissProt: Q9UHC1 Human
Unigene: 436650 Human
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Immunocytochemistry/ Immunofluorescence - Anti-MLH3 antibody (ab4834)
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab4834 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).