Anti-IRS1 (phospho S616)抗体
参阅全部 IRS1 一抗
兔多克隆抗体to IRS1 (phospho S616)
Rabbit
适用于: IHC-P, WBmore details
与反应: Mouse, Human
预测可用于: Rat
Synthetic peptide corresponding to IRS1 (phospho S616).
WB: HEK-293T cells. IHC-P: Human breast carcinoma tissue, mouse skeletal muscle tissue.
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Liquid
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA, 50% Glycerol
BSA is IgG and protease free
Immunogen affinity purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated insulin receptor substrate 1 (IRS 1).
多克隆
IgG
Abpromise™承诺保证使用ab4776于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/10 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000. Predicted molecular weight: 165 kDa. |
Entrez Gene: 3667 Human
Entrez Gene: 16367 Mouse
Omim: 147545 Human
SwissProt: P35568 Human
SwissProt: P35569 Mouse
Unigene: 471508 Human
Unigene: 4952 Mouse
Unigene: 10476 Rat
HIRS 1 antibody
HIRS1 antibody
Insulin receptor substrate 1 antibody
IRS 1 antibody
IRS-1 antibody
IRS1 antibody
IRS1_HUMAN antibody
OTTHUMP00000164234 antibody
Western blot - Anti-IRS1 (phospho S616) antibody (ab4776)
Extracts of 293T cells transfected with wild-type human IRS1 and treated with 100 ng/mL TPA for 30 minutes were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 (phospho S616) antibody (ab4776)
Immunohistochemistry analysis of IRS1 (phospho S616) showing staining in the nucleus of paraffin-embedded human breast carcinoma tissue (right) compared to a negative control without primary antibody (left). Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab4776 diluted in 3% BSA-PBS at a dilution of 1/100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 (phospho S616) antibody (ab4776)
Immunohistochemistry analysis of IRS1 (phospho S616) showing staining in the nucleus of paraffin-embedded mouse skeletal muscle tissue (right) compared to a negative control without primary antibody (left). Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab4776 diluted in 3% BSA-PBS at a dilution of 1/20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
