Anti-Tau (phospho S409)抗体
参阅全部 Tau 一抗
兔多克隆抗体to Tau (phospho S409)
Rabbit
Phosphorylation site-specific antibody selective for the phosphorylated form of human tau containing a phosphate on serine 409.
The specificity of this antibody refers to P10636-8.
适用于: IHC-P, WBmore details
与反应: Mouse, Recombinant fragment
预测可用于: Rat
Synthetic peptide corresponding to Human Tau (phospho S409). Synthetic peptide (Human) derived from a region of human tau that contains serine 409. The sequence is conserved in mouse and rat.
Database link: P10636-8
WB: Recombinant human Tau left untreated, or treated with PKA. IHC: Mouse brain tissue.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Immunogen affinity purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated tau. The final product is generated by affinity chromatography using a tau-derived peptide that is phosphorylated at serine 409.
多克隆
IgG
Abpromise™承诺保证使用ab4861于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/10 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000. Predicted molecular weight: 62 kDa. |
Entrez Gene: 17762 Mouse
SwissProt: P10637 Mouse
Unigene: 1287 Mouse
Unigene: 2455 Rat
There are 9 isoforms produced by alternative splicing.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S409) antibody (ab4861)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue labelling Tau with ab4873 at 1/20 dilution. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature and washed with ddH2O and PBS. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Western blot - Anti-Tau (phospho S409) antibody (ab4861)
WB using ab4861. The peptide corresponding to Tau [pS409] blocks the antibody signal, thereby demonstrating the specificity of the antibody.
WB using ab4861. The peptide corresponding to Tau [pS409] blocks the antibody signal, thereby demonstrating the specificity of the antibody.
