Anti-Src (phospho Y419)抗体
参阅全部 Src 一抗
兔多克隆抗体to Src (phospho Y419)
Rabbit
适用于: IHC-P, IHC-FoFr, WBmore details
与反应: Mouse, Chicken, Human
Synthetic peptide corresponding to Src (phospho Y419). The sequence is conserved in mouse, rat, chicken and frog.
Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418 (tyrosine 414 on frog Src, tyrosine 416 on chicken Src, and tyrosine 424 on mouse Src). This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
浓度
50 µl 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively pre-adsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Src, and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a Src-derived peptide that is phosphorylated at tyrosine 418.
Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418 (tyrosine 414 on frog Src, tyrosine 416 on chicken Src, and tyrosine 424 on mouse Src). This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.
多克隆
IgG
Abpromise™承诺保证使用ab4816于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/10 - 1/100. | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 21901128 | |
| WB | (2) | 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). |
Entrez Gene: 6714 Human
Entrez Gene: 20779 Mouse
Omim: 190090 Human
SwissProt: P00523 Human
SwissProt: P12931 Human
SwissProt: P05480 Mouse
Unigene: 195659 Human
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
c SRC antibody
CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody
cSrc antibody
EC 2.7.10.2 antibody
Neuronal CSRC tyrosine specific protein kinase antibody
Neuronal proto-oncogene tyrosine-protein kinase Src antibody
Neuronal SRC antibody
Oncogene SRC antibody
OTTHUMP00000174476 antibody
OTTHUMP00000174477 antibody
p60 Src antibody
p60-Src antibody
p60c-src antibody
p60Src antibody
pp60c src antibody
pp60c-src antibody
pp60csrc antibody
Proto oncogene tyrosine protein kinase Src antibody
Proto-oncogene c-Src antibody
Proto-oncogene tyrosine-protein kinase Src antibody
Protooncogene SRC antibody
Protooncogene SRC Rous sarcoma antibody
Src antibody
SRC Oncogene antibody
SRC proto oncogene non receptor tyrosine kinase antibody
SRC_HUMAN antibody
SRC1 antibody
Tyrosine kinase pp60c src antibody
Tyrosine protein kinase SRC 1 antibody
Tyrosine protein kinase SRC1 antibody
v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody
V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibody
v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody
ASV antibody
Avian sarcoma virus antibody
AW259666 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src (phospho Y418) antibody (ab4816)
Immunohistochemical analysis of paraffin embedded Human colon carcinoma, labelling Src with ab4816. A negative control without ab4816 is shown on the left. Antigen retrieval performed using 10mM sodium citrate, microwaved for 8-15mins, blocked using 3% H2O2-methanol, then treated with ab4816 followed by an HRP-conjugated secondary antibody. Counterstaining with hematoxylin.
Western blot - Anti-Src (phospho Y418) antibody (ab4816)Dr. Michael Schaller, Dept. of Cell Bio. & Anat., Univ. of North Carolina, Chapel Hill, NC.
Western blot detection of Src phosphorylation in extracts of chick embryo fibroblasts expressing wild-type (lanes 2,4,6) or mutant (lanes 1,3,5) pp60src. Truncation of Src at position 518 eliminated phosphorylation at the negative regulatory site [pY529], while increasing phosphorylation at the catalytic site [pY418].
Western blot detection of Src phosphorylation in extracts of chick embryo fibroblasts expressing wild-type (lanes 2,4,6) or mutant (lanes 1,3,5) pp60src. Truncation of Src at position 518 eliminated phosphorylation at the negative regulatory site [pY529], while increasing phosphorylation at the catalytic site [pY418].
