Anti-alpha Tubulin抗体- Loading Control
参阅全部 alpha Tubulin 一抗
兔多克隆抗体to alpha Tubulin - Loading Control
Rabbit
This antibody detects a single band in cell lines, corresponding to alpha tubulin.
适用于: WB, ICC/IFmore details
与反应: Mouse, Rat, Chicken, Cow, Human, Chinese hamster
预测可用于: Pig, Xenopus laevis, Chimpanzee, Monkey, Zebrafish
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
WB: HeLa, NIH 3T3, and PC12 cell lysates. ICC/IF: HeLa cells. IHC-P: Human endometrium tissue.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.9 - 1 mg/ml
批次浓度范围 250 µg 浓度为 0.6 - 1 mg/ml
Immunogen affinity purified
This antibody makes an excellent loading control.
多克隆
IgG
Abpromise™承诺保证使用ab4074于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (18) | Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). Can be blocked with alpha Tubulin peptide (ab23537). |
| ICC/IF | (9) | Use a concentration of 1 µg/ml. |
Entrez Gene: 7277 Human
Entrez Gene: 22145 Mouse
Entrez Gene: 573122 Zebrafish
Entrez Gene: 573216 Zebrafish
Omim: 191110 Human
SwissProt: P68366 Human
SwissProt: P68368 Mouse
Unigene: 75318 Human
Unigene: 1155 Mouse
Unigene: 92961 Rat
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Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody - Loading Control (ab4074)
ab4074 staining alpha Tubulin in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab4064 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-alpha Tubulin antibody - Loading Control (ab4074)
All lanes : Anti-alpha Tubulin antibody - Loading Control (ab4074) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
Lane 2 : NIH/3T3 (Mouse) Whole Cell Lysate (ab52956)
Lane 3 : PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate (ab50957)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody - Loading Control (ab4074)Image from PLoS One. 2014; 9(10): e109590. Fig 5o,doi: 10.1371/journal.pone.0109590 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunocytochemistry of SisNPFR-C6E8 cell line. Cells were prefixed in freshly prepared 4% paraformaldehyde (PFA) in F12 K culture medium with 10% fetal bovine serum and Geneticin. Fixed cells were permeabilized in PBST (0.25% Triton X-100 in PBS) for 5 min and blocked at 4°C overnight in 5% normal goat serum. The cells were then incubated at 4°C overnight with ab4074 (1:50). Cover slips were placed onto slides and mounted with VectaShield containing DAPI.
Western blot - Anti-alpha Tubulin antibody - Loading Control (ab4074)
All lanes : Anti-alpha Tubulin antibody - Loading Control (ab4074) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
Lane 2 : HEK-293 (Human) Whole Cell Lysate (ab52256)
Lane 3 : NIH/3T3 whole cell lysate (ab7179)
Lane 4 : PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate (ab50957)
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) with Human alpha Tubulin peptide (ab23537) at 1 µg/ml
Lane 6 : HEK-293 (Human) Whole Cell Lysate (ab52256) with Human alpha Tubulin peptide (ab23537) at 1 µg/ml
Lane 7 : NIH/3T3 whole cell lysate (ab7179) with Human alpha Tubulin peptide (ab23537) at 1 µg/ml
Lane 8 : PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate (ab50957) with Human alpha Tubulin peptide (ab23537) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Western blot - Anti-alpha Tubulin antibody - Loading Control (ab4074)
All lanes : Anti-alpha Tubulin antibody - Loading Control (ab4074) at 1 µg/ml
Lane 1 : Mouse 3T3 lysate
Lane 2 : Rat liver lysate
Lane 3 : Cow cell lysate
Lane 4 : Chicken cell lysate
Lane 5 : CHO lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 30 seconds
ab4074 cross-reacts strongly with Mouse 3T3 lysate and shows weak cross-reactivity with Rat liver, Cow, Chicken and CHO lysates.
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