Anti-alpha 1 Fetoprotein抗体[AFP-01]
参阅全部 alpha 1 Fetoprotein 一抗
小鼠单克隆抗体[AFP-01] to alpha 1 Fetoprotein
Mouse
The antibody reacts with human alpha-Fetoprotein (AFP), an oncofetal antigen of 70 kDa. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
适用于: ICC/IFmore details
与反应: Human
Full length native protein (purified) corresponding to Human alpha 1 Fetoprotein.
ICC/IF: MCF7 cells
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS
Proprietary Purification
Purified by precipitation and chromatography
单克隆
AFP-01
unknown
IgG1
unknown
Abpromise™承诺保证使用ab3980于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) | Use a concentration of 5 µg/ml. |
Entrez Gene: 174 Human
Omim: 104150 Human
SwissProt: P02771 Human
Unigene: 518808 Human
Alpha fetoprotein antibody
Alpha fetoprotein precursor antibody
Alpha-1-fetoprotein antibody
Alpha-fetoglobulin antibody
Alpha-fetoprotein antibody
alpha-fetoprotein, Hereditary persistence of, included antibody
FETA antibody
FETA_HUMAN antibody
Hereditary persistence of alpha fetoprotein antibody
HPAFP antibody
Afp antibody
AFPD antibody
Alpha fetoglobulin antibody
![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)](https://www.abcam.cn/ps/products/3/ab3980/Images/ab3980-8451-anti-alpha-1-fetoprotein-antibody-afp-01-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)
ICC/IF image of ab3980 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3980, 5µg/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
