Anti-FAK (phospho Y407)抗体
参阅全部 FAK 一抗
兔多克隆抗体to FAK (phospho Y407)
Rabbit
Phosphorylation site-specific antibody selective for the phosphorylated form of the Focal Adhesion Kinase enzyme containing a phosphate on tyrosine 407. The antibody has been shown to recognize Focal Adhesion Kinase (approximately 125 kDa) in chick embryo fibroblast cells plated on fibronectin.
适用于: WBmore details
与反应: Chicken
Synthetic peptide corresponding to FAK (phospho Y407). The sequence is conserved in mouse, rat and chicken.
Focal Adhesion Kinase is a non-receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signalling. Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. The activity of the phosphorylation site pTyr407 is currently unknown.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Immunogen affinity purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Focal Adhesion Kinase enzyme and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a Focal Adhesion Kinase-derived peptide that is phosphorylated at tyrosine 407.
Focal Adhesion Kinase is a non-receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signalling. Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. The activity of the phosphorylation site pTyr407 is currently unknown.
多克隆
IgG
Abpromise™承诺保证使用ab4814于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 125 kDa. |
Entrez Gene: 396416 Chicken
SwissProt: Q00944 Chicken
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Western blot - Anti-FAK (phospho Y407) antibody (ab4814)
Cell extracts prepared from chick embryo fibroblasts expressing FAK and plated on fibronectin were resolved by SDS-PAGE on a 4-20% Tris-glycine gel. The proteins were then transferred to nitrocellulose. Membranes were incubated with 0.3 µg/mL ab4814, following prior incubation in the absence (none) or presence of the peptide immunogen, the non-phosphopeptide corresponding to the FAK phosphopeptide, or a generic phosphotyrosine peptide. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, therefore demonstrating the specificity of ab4814 for this phosphorylated residue. Cell extracts prepared from chick embryo fibroblasts expressing FAK and plated on fibronectin were resolved by SDS-PAGE on a 4-20% Tris-glycine gel. The proteins were then transferred to
