Anti-Vimentin抗体[VI-RE/1]
参阅全部 Vimentin 一抗
小鼠单克隆抗体[VI-RE/1] to Vimentin
Mouse
The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament protein expressed on a wide variety of mesenchymal and mesodermal cell types.
适用于: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details
与反应: Human
预测可用于: Rat
不与反应: Mouse, Pig
Recombinant full length protein corresponding to Human Vimentin aa 1 to the C-terminus.
Database link: P08670
ICC: HeLa cells. IHC-P: Human bladder and liver tissue. Flow Cyt (Intra): Human ESS-1 and HeLa cells. WB: HeLa whole cell lysate.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS
浓度
100 µg 浓度为 1 mg/ml
Protein A purified
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.
单克隆
VI-RE/1
IgG1
Abpromise™承诺保证使用ab3974于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) | Use a concentration of 1 - 4 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. | |
| ICC/IF | Use a concentration of 5 - 10 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. Detects a band of approximately 57 kDa. Positive control: LEP-19 cell lysate Negative control: 3T3 mouse cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 3 min in water bath. Read More | |
| IHC-P | (1) | 1/400. |
Entrez Gene: 7431 Human
Omim: 193060 Human
SwissProt: P08670 Human
Unigene: 455493 Human
Unigene: 691131 Human
Unigene: 2710 Rat
Vimentin is found in connective tissue and in the cytoskeleton.
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![Flow Cytometry (Intracellular) - Anti-Vimentin antibody [VI-RE/1] (ab3974)](https://www.abcam.cn/ps/products/3/ab3974/Images/ab3974-455618-anti-vimentin-antibody-vi-re-1-flow-cytometry-intracellular-ess-cells-human.jpg)
Flow Cytometry (Intracellular) - Anti-Vimentin antibody [VI-RE/1] (ab3974)
Flow cytometry (Intracellular) analysis ESS-1 (human endometrial stromal sarcoma cell line) cells labeling Vimentin with ab3974 (GAM-FITC).
Negative control: human lymphocytes.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [VI-RE/1] (ab3974)](https://www.abcam.cn/ps/products/3/ab3974/Images/ab3974-471164-ab3974-anti-vimentin-antibody-human-liver-immunocytochemistry.png)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [VI-RE/1] (ab3974)
Immunohistochemistry staining (paraffin sections) of Vimentin in human liver using mouse monoclonal antibody [VI-RE/1] (ab3974, diluted 1/400), detected with GAM IgG-Alexa Fluor®488 (diluted 1/200; green), cell nuclei stained with PI (1 µg/ml; orange).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [VI-RE/1] (ab3974)](https://www.abcam.cn/ps/products/3/ab3974/Images/ab3974-210351-anti-vimentin-antibody-vi-re-1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [VI-RE/1] (ab3974)
IHC image of Vimentin staining in human bladder formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3974, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [VI-RE/1] (ab3974)](https://www.abcam.cn/ps/products/3/ab3974/Images/ab3974-8444-anti-vimentin-antibody-vi-re-1-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [VI-RE/1] (ab3974)
ICC image of ab3974 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3974, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Flow Cytometry (Intracellular) - Anti-Vimentin antibody [VI-RE/1] (ab3974)](https://www.abcam.cn/ps/products/3/ab3974/Images/ab3974-8445-anti-vimentin-antibody-vi-re-1-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Vimentin antibody [VI-RE/1] (ab3974)
Flow Cytometry (Intracellular) analysis of HeLa cells labeling Vimentin with ab3974 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3974, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
![Western blot - Anti-Vimentin antibody [VI-RE/1] (ab3974)](https://www.abcam.cn/ps/products/3/ab3974/Images/ab3974-8446-anti-vimentin-antibody-vi-re-1-western-blot.jpg)
Western blot - Anti-Vimentin antibody [VI-RE/1] (ab3974)
Anti-Vimentin antibody [VI-RE/1] (ab3974) at 5 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size: 57 kDawhy is the actual band size different from the predicted?
