Anti-Nsr1p抗体[31C4]
小鼠单克隆抗体[31C4] to Nsr1p
Mouse
This antibody was isolated during a screen for mAbs against yeast nucleolar proteins. Specificity was determined by screening a yeast genomic library in lambda gt11. Two individually isolated, different positive clones were found. Sequencing revealed that in both clones beta-galactosidase was fused at the EcoRI site to the amino acid sequence beginning with …EFEH190 in Nsr1p. Thus, it is likely to react with an epitope(s) in the C-terminal 55% of Nsr1p.
适用于: ICC/IF, WBmore details
与反应: Saccharomyces cerevisiae
Tissue, cells or virus corresponding to Nsr1p. Yeast nuclear preparation
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant
Tissue culture supernatant
Sterile filtered.
单克隆
31C4
IgG
Abpromise™承诺保证使用ab4642于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) | 1/500 - 1/5000. (yeast cells). |
| WB | (1) | 1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 45 kDa). For non-ECL western detection methods, 1/1000 - 1/5000. |
Nuclear
Entrez Gene: 853064 Saccharomyces cerevisiae
SwissProt: P27476 Saccharomyces cerevisiae
NSR1 antibody
Nuclear localization sequence binding protein antibody
p67 antibody
SHE5 antibody
![Western blot - Anti-Nsr1p antibody [31C4] (ab4642)](https://www.abcam.cn/ps/products/4/ab4642/Images/Nsr1p-Primary-antibodies-ab4642-5.jpg)
Western blot - Anti-Nsr1p antibody [31C4] (ab4642)Image courtesy of an anonymous Abreview.
All lanes : Anti-Nsr1p antibody [31C4] (ab4642) at 1/10000 dilution
Lane 1 : Saccharomyces cerevisae DDY1810 (WT) whole cell lysate
Lane 2 : Saccharomyces cerevisae DDY1810 (nsr1 knockout) whole cell lysate
Secondary
All lanes : Rabbit anti-mouse IgG conjugated to peroxidase at 1/30000 dilution
Performed under reducing conditions.
Predicted band size: 45 kDa
Bottom panel: loading control performed using Saccharomyces cerevisae anti-HSP60 (1:1000)
![Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)](https://www.abcam.cn/ps/products/4/ab4642/Images/Nsr1p-Primary-antibodies-ab4642-7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)Image courtesy of an anonymous Abreview.
ab4642 (1/5000) detecting Nsr1p in DDY1810 (red). Cells were fixed in paraformaldehyde, permeabilized with methanol/acetone and counterstained with DAPI in order to highlight the nucleus (blue).
Right hand panel: negative control performed using Saccharomyces cerevisae DDY1810 nsr1p knockout.
For further experimental details please refer to Abreview.
