Anti-hnRNP L antibody [4D11]

• 产品名称

  Anti-hnRNP L抗体[4D11]
  参阅全部 hnRNP L 一抗

• 描述

  小鼠单克隆抗体[4D11] to hnRNP L

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, WB, IP, Flow Cytmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Human hnRNP proteins (from HeLa cells) purified by affinity chromatography on ssDNA agarose.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.1% Sodium azide
  Constituent: PBS

• 浓度

• 100 µl 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Purified from supernatant.

• 克隆

  单克隆

• 克隆编号

  4D11

• 骨髓瘤

  Sp2/0

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab6106于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3191 Human

• Omim: 603083 Human

• SwissProt: P14866 Human

• Unigene: 644906 Human

• 别名

• hnRNP L antibody

• hnRNP-L antibody

• Hnrnpl antibody

• hnRPL antibody

• HNRPL_HUMAN antibody

• P/OKcl.14 antibody

• D830027H13Rik antibody

• FLJ35509 antibody

• Heterogeneous nuclear ribonucleoprotein L antibody

• 

  Western blot - Anti-hnRNP L antibody [4D11] (ab6106)

  All lanes : Anti-hnRNP L antibody [4D11] (ab6106) at 1 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
  Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 64 kDa
  Observed band size: 64 kDa
  Additional bands at: 60 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 1 minute
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP L antibody [4D11] (ab6106)

  IHC image of ab6106 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6106, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  
  

• 

  Immunoprecipitation - Anti-hnRNP L antibody [4D11] (ab6106)

  hnRNP L was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to hnRNP L (ab6106) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
  The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
  Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab6106.
  Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
  Band: 65kDa: hnRNP L. Non specific - 70kDa and 55kDa: We are unsure as to the identity of this extra band.

• 

  Flow Cytometry - Anti-hnRNP L antibody [4D11] (ab6106)

  Overlay histogram showing Jurkat cells stained with ab6106 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6106, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.