Anti-CREBBP抗体
参阅全部 CREBBP 一抗
兔多克隆抗体to CREBBP
Rabbit
适用于: WB, IHC-P, ICC/IFmore details
与反应: Mouse, Human
预测可用于: Non human primates
Synthetic peptide corresponding to Human CREBBP aa 162-176.
Sequence:
ATSSPATSQTGPGIC
(Peptide available as ab4916)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators that have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB (cyclic AMP-responsive enhancer binding protein), c-Fos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD.Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes.As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.Single point mutations in CBP have been proposed as causative factors in the developmental abnormalities of Rubinstein-Taybi syndrome (RTS).Although both CBP and p300 appear to function similarly, the inability of p300 to rescue CBP malfunction iRTS suggests intrinsic functional differences between CBP and p300.
多克隆
IgG
Abpromise™承诺保证使用ab2832于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 265 kDa (predicted molecular weight: 265 kDa). |
| IHC-P | (1) | 1/1000 - 1/5000. |
| ICC/IF | 1/100. |
Entrez Gene: 1387 Human
Entrez Gene: 12914 Mouse
Omim: 600140 Human
SwissProt: Q92793 Human
SwissProt: P45481 Mouse
Unigene: 459759 Human
Unigene: 132238 Mouse
Unigene: 392384 Mouse
CREB-binding protein antibody
Crebbp antibody
Cyclic AMP responsive enhancer binding protein antibody
KAT3A antibody
RSTS antibody
RTS antibody
Rubinstein Taybi syndrome antibody
CBP antibody
CBP_HUMAN antibody
CREB binding protein antibody
Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody (ab2832)
Immunofluorescent analysis of MCF-7 cells, labeling CREBBP with ab2832 (right) compared with a negative control without ab2832 (left). Cells were formalin fixed, permeabilized with 0.1% Triton X-100 for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with anti-KAT3A/CBP diluted 1/100 in 3% BSA/PBS overnight at 4°C. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with DAPI (blue). The nuclear localization of KAT3A/CBP can be observed (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody (ab2832)
IHC image of CREBBP staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2832, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody (ab2832)
Immunofluorescent analysis of HeLa cells, labeling CREBBP with ab2832 (right) compared with a negative control without ab2832 (left). Cells were formalin fixed, permeabilized with 0.1% Triton X-100 for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with anti-KAT3A/CBP diluted 1/100 in 3% BSA/PBS overnight at 4°C. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with DAPI (blue). The nuclear localization of KAT3A/CBP can be observed (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody (ab2832)
Immunohistochemical analysis of FFPE mouse colon tissue, labeling CREBBP with ab2832 (right) compared with a negative control without ab2832 (left). Heat antigen retrieval performed with 10mM Sodium Citrate (pH 6) for 8-15 minutes. Tissue blocked with 3% H2O2-methanol for 15 minutes at room temperature. Incubation with ab2832 diluted 1/2000 in 3% BSA-PBS overnight at 4°C. Counterstaining with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody (ab2832)
Immunofluorescent analysis of NIH-3T3 cells, labeling CREBBP with ab2832 (right) compared with a negative control without ab2832 (left). Cells were formalin fixed, permeabilized with 0.1% Triton X-100 for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with anti-KAT3A/CBP diluted 1/100 in 3% BSA/PBS overnight at 4°C. Nuclei were stained with DAPI (blue).
Western blot - Anti-CREBBP antibody (ab2832)
ab2832 using HeLa cell lysate. ab2832 using HeLa cell lysate.
