Anti-beta Catenin抗体[15B8]
参阅全部 beta Catenin 一抗
小鼠单克隆抗体[15B8] to beta Catenin
Mouse
适用于: WBmore details
与反应: Mouse, Rat, Human
预测可用于: Cow, Dog, Zebrafish
Recombinant full length protein corresponding to Chicken beta Catenin.
WB: Hap1, HeLa, A43, HEK293, Caco2, NIH3T3 and PC12 cell lysates.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Affinity purified
单克隆
15B8
IgG1
kappa
Abpromise™承诺保证使用ab6301于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | Use a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 85 kDa). |
Entrez Gene: 1499 Human
Entrez Gene: 12387 Mouse
Entrez Gene: 30265 Zebrafish
Omim: 116806 Human
SwissProt: P35222 Human
SwissProt: Q02248 Mouse
Unigene: 476018 Human
Unigene: 291928 Mouse
Unigene: 112601 Rat
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![Western blot - Anti-beta Catenin antibody [15B8] (ab6301)](https://www.abcam.cn/ps/products/6/ab6301/Images/ab6301-284316-anti-beta-catenin-antibody-15b8-western-blot.jpg)
Western blot - Anti-beta Catenin antibody [15B8] (ab6301)
All lanes : Anti-beta Catenin antibody [15B8] (ab6301) at 1 µg/ml
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : CTNNB1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 85 kDa
Lanes 1 - 3: Merged signal (red and green). Green - ab6301 observed at 85 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab6301 was shown to specifically react with beta-Catenin in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when knockout samples were used. Wild-type and beta Catenin knockout samples were subjected to SDS-PAGE. Ab6301 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1 µg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
![Western blot - Anti-beta Catenin antibody [15B8] (ab6301)](https://www.abcam.cn/ps/products/6/ab6301/Images/ab6301-12207-anti-beta-catenin-antibody-15b8-western-blot.jpg)
Western blot - Anti-beta Catenin antibody [15B8] (ab6301)
All lanes : Anti-beta Catenin antibody [15B8] (ab6301) at 1 µg/ml
Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 95 kDawhy is the actual band size different from the predicted?
Additional bands at: 73 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab6301 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
