Anti-MRP3 antibody [M3II-9]

• 产品名称

  Anti-MRP3抗体[M3II-9]
  参阅全部 MRP3 一抗

• 描述

  小鼠单克隆抗体[M3II-9] to MRP3

• 宿主

  Mouse

• 特异性

  This antibody does not cross react with MDR1, MRP1, MRP2 or MRP5 gene products

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant fragment within Human MRP3 aa 800-950. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
  Database link: O15438-1

  Run BLAST with Run BLAST with 

• 表位

  This antibody detects with an internal epitope of the membrane transport protein of MRP3

• 阳性对照

• Cell lines: Drug sensitive parental cell lines and their multidrug resistant derivatives

• 常规说明

  The rat species was added as a result of positive Abreviews and publication PMID 22112382. In response to few recent complaints, we have decided to remove this species and no longer guarantee it. Please contact technical@abcam.com for any inquiry.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.3
  Constituent: 0.7% BSA

• 纯度

  Tissue culture supernatant

• 克隆

  单克隆

• 克隆编号

  M3II-9

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab3375于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 8714 Human

• Omim: 604323 Human

• SwissProt: O15438 Human

• Unigene: 463421 Human

• 别名

• ABCC3 antibody

• ATP binding cassette sub family C (CFTR/MRP) member 3 antibody

• ATP binding cassette sub family C member 3 antibody

• ATP-binding cassette sub-family C member 3 antibody

• Canalicular multispecific organic anion transporter 2 antibody

• Canicular multispecific organic anion transporter antibody

• CMOAT 2 antibody

• CMOAT2 antibody

• EST90757 antibody

• MLP 2 antibody

• MLP2 antibody

• MOAT D antibody

• MOAT-D antibody

• MOATD antibody

• MRP 3 antibody

• MRP3_HUMAN antibody

• Multi specific organic anion transporter D antibody

• Multi-specific organic anion transporter D antibody

• Multidrug resistance associated protein 3 antibody

• Multidrug resistance associated protein antibody

• Multidrug resistance-associated protein 3 antibody

• Multispecific organic anion tranporter D antibody

• ABC 31 antibody

• ABC31 antibody

• ABCC 3 antibody

• 

  Western blot - Anti-MRP3 antibody [M3II-9] (ab3375)

   HepG2 cells were incubated at 37°C for 24h with vehicle control (0 μM) and different concentrations of clotrimazole (ab120744) in DMSO. Increased expression of MRP3 (ab3375) correlates with an increase in clotrimazole concentration, as described in literature.

  Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab3375 at 1/50 dilution and ab9484 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.