Anti-DAP3抗体
参阅全部 DAP3 一抗
山羊多克隆抗体to DAP3
Goat
This antibody is expected to recognise both human isoforms of this protein.
适用于: ICC/IF, WBmore details
与反应: Human
Synthetic peptide corresponding to Human DAP3 aa 387-398 (C terminal).
Sequence:
NPSLLERHCAYL
(Peptide available as ab22922)
WB: HeLa and HepG2 cell lysate and human kidney tissue lysate. ICC/IF: MCF7 cells.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab2637于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 3 - 10 µg/ml. | |
| WB | Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 46 kDa. 1 hour primary incubation is recommended for this product. |
Entrez Gene: 7818 Human
Omim: 602074 Human
SwissProt: P51398 Human
Unigene: 516746 Human
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Western blot - Anti-DAP3 antibody (ab2637)
Anti-DAP3 antibody (ab2637) at 0.3 µg/ml + Human kidney tissue lysate in RIPA buffer at 30 µg
Predicted band size: 46 kDa
Detected using chemiluminescence.
Immunocytochemistry/ Immunofluorescence - Anti-DAP3 antibody (ab2637)
Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labelling DAP3 with ab2637 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
Western blot - Anti-DAP3 antibody (ab2637)
All lanes : Anti-DAP3 antibody (ab2637) at 0.3 µg/ml
Lane 1 : HeLa cell lysate in RIPA buffer
Lane 2 : HepG2 cell lysate in RIPA buffer
Lysates/proteins at 30 µg per lane.
Predicted band size: 46 kDa
Detected using chemiluminescence.
Western blot - Anti-DAP3 antibody (ab2637)
All lanes : Anti-DAP3 antibody (ab2637) at 1 µg/ml (for 1 hour)
Lane 1 : HeLa cell lysate in RIPA buffer - control
Lane 2 : HeLa cell lysate in RIPA buffer - after siRNA mediated DAP3 knock-down expression
Lysates/proteins at 35 µg per lane.
Predicted band size: 46 kDa
Level of knock-down relative to actin expression level determined by RT-PCR.
