Anti-Collagen III antibody [FH-7A]

• 产品名称

  Anti-Collagen III抗体[FH-7A]
  参阅全部 Collagen III 一抗

• 描述

  小鼠单克隆抗体[FH-7A] to Collagen III

• 宿主

  Mouse

• 特异性

  ab6310 specifically recognizes collagen type III from human and rat origin. It does not recognize collagen types I, II, IV, V, VI and X.

• 经测试应用

  适用于: IHC-Fr, IHC-P, WB, ELISA, Dot blot, ICC/IFmore details

• 种属反应性

  与反应: Rat, Human
  

• 免疫原

  Full length native protein (purified) (Human).

• 阳性对照

• IHC-P: Rat skin sections. IHC-Fr: Rat skin sections.

• 常规说明

  Type III collagen, [a1(III)]3 ,is an approx. 300 kDa molecule, found predominantly in skin, blood vessels, liver, placenta, tongue, and thymus. Collagen type III forms cofibrils with type I and/or V collagens in a number of tissues of mesenchymal origin, such as skin, tendon, ligaments, and bone. This collagen type is involved, directly or indirectly in several genetic diseases, including Ehlers-Danlos type IV disease.

   

  This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.097% Sodium azide
  Constituent: Whole serum

• 纯度

  Tissue culture supernatant

• 克隆

  单克隆

• 克隆编号

  FH-7A

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab6310于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 1281 Human

• Entrez Gene: 84032 Rat

• Omim: 120180 Human

• SwissProt: P02461 Human

• SwissProt: P13941 Rat

• Unigene: 443625 Human

• Unigene: 3247 Rat

• 别名

• COL 3A1 antibody

• COL3A1 antibody

• Collagen alpha 1(III) chain antibody

• Collagen alpha-1(III) chain antibody

• Collagen III alpha 1 chain precursor antibody

• Collagen III alpha 1 polypeptide antibody

• Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody

• Collagen type III alpha 1 antibody

• Collagen type III alpha 1 chain antibody

• Collagen type III alpha antibody

• Collagen, fetal antibody

• EDS4A antibody

• Ehlers Danlos syndrome type IV, autosomal dominant antibody

• Fetal collagen antibody

• Type III collagen antibody

• Alpha 1 type III collagen antibody

• Alpha1 (III) collagen antibody

• CO3A1_HUMAN antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)

  Staining of formalin-fixed, paraffin-embedded rat skin with 1:4,000 ab6310 using biotin/ExtrAvidin®-Peroxidase.

  This image was generated using the ascites version of the product.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)This image is courtesy of an Abreview submitted by Birgitta Weijdegard

  ab6310 at 1/600 diltuion staining preovulatory follicle and whole ovary tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigens were retrieved by boiling with an antigen unmasking solution for 20 min at 120ºC in an autoclave and then cooled down in water for 5 minutes. The tissue sections were formaldehyde fixed and incubated with the antibody for 1 hour. An alkaline phosphatase conjugated antibody was used as the secondary. The image shows a section of whole preovulatory follicle. Staining for collagen type III is seen in the theca interna cell layer. No staining in the granulosa cells.

  This image was generated using the ascites version of the product.

  See Abreview

• 

  Immunohistochemistry (Frozen sections) - Anti-Collagen III antibody [FH-7A] (ab6310)

  Staining of frozen rat skin sections with 1:8,000 ab6310 using biotin/ExtrAvidin®-Peroxidase.

  This image was generated using the ascites version of the product.