Anti-Grp75/MOT antibody [JG1]

• 产品名称

  Anti-Grp75/MOT抗体[JG1]
  参阅全部 Grp75/MOT 一抗

• 描述

  小鼠单克隆抗体[JG1] to Grp75/MOT

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, IP, IHC-P, Flow Cyt, WBmore details

• 种属反应性

  与反应: Mouse, Rat, Guinea pig, Hamster, Dog, Human, Non human primates
  预测可用于: Cow

• 免疫原

  Synthetic peptide corresponding to Mouse Grp75/MOT aa 661-679.
  Sequence:

  GSGSSGTGEQKEDQKEEKQ

  
  Database link: P38647

  Run BLAST with Run BLAST with 

• 阳性对照

• Recombinant Human Grp75/MOT protein (ab79145) can be used as a positive control in WB. IHC-P: Human testis tissue. ICC/IF: A549, HMVEC, NS-1, P19, HeLa and human fibroblasts cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: PBS, BSA

• 浓度

• 100 µl 浓度为 1.07 mg/ml

• 纯度

  Protein A purified

• Primary antibody说明

  The HSP 70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP 70 family members include: HSP 70, a 70 kDa protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP 72, a 72 kDa protein which is induced exclusively under stress conditions; HSC 70, or cognate protein, is a 72 kDa constitutively expressed protein which is involved in uncoating clathrin-coated vesicles; GRP 78, or BiP, is a glucose-regulated 78 kDa protein localized to the endoplasmic reticulum; and mitochondrial HSP 70 (mtHSP 70, GRP 75 or mortalin) a 75 kDa protein that is found within the mitochondria. mtHSP 70 is a mitochondrial resident protein that is involved in protein translocation into the mitochondria. Preproteins cross the mitochondrial membranes in an extended conformation. This requires unfolding of preproteins before entering translocation pores in the mitochondrial outer membrane. Preprotein unfolding is thought to be mediated by mtHSP 70 and other inner membrane proteins of the mitochondria.

• 克隆

  单克隆

• 克隆编号

  JG1

• 同种型

  IgG3

The Abpromise guarantee

Abpromise™承诺保证使用ab2799于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 517535 Cow

• Entrez Gene: 474697 Dog

• Entrez Gene: 101837753 Hamster

• Entrez Gene: 3313 Human

• Entrez Gene: 15526 Mouse

• Entrez Gene: 291671 Rat

• Omim: 600548 Human

• SwissProt: Q3ZCH0 Cow

• SwissProt: P99502 Dog

• SwissProt: P86233 Hamster

• SwissProt: P38646 Human

• SwissProt: P38647 Mouse

• SwissProt: P48721 Rat

• Unigene: 184233 Human

• Unigene: 209419 Mouse

• Unigene: 7535 Rat

别名

• 75 kDa glucose regulated protein antibody

• 75 kDa glucose-regulated protein antibody

• CSA antibody

• Glucose Regulated Protein antibody

• Grp 75 antibody

• GRP-75 antibody

• GRP75 antibody

• GRP75_HUMAN antibody

• Heat shock 70 kDa protein 9 antibody

• Heat shock 70kD protein 9 antibody

• heat shock 70kDa protein 9 antibody

• Heat shock 70kDa protein 9B antibody

• Heat shock protein 74 kDa A antibody

• Heat shock protein A antibody

• Heat shock protein cognate 74 antibody

• Hsc74 antibody

• Hsp74 antibody

• Hsp74a antibody

• HSPA9 antibody

• Hspa9a antibody

• HSPA9B antibody

• MGC4500 antibody

• mitochondrial antibody

• Mortalin 2 antibody

• Mortalin antibody

• Mortalin perinuclear antibody

• Mortalin2 antibody

• MOT 2 antibody

• MOT antibody

• MOT2 antibody

• Mthsp70 antibody

• p66 mortalin antibody

• P66 MOT antibody

• PBP74 antibody

• Peptide binding protein 74 antibody

• Peptide-binding protein 74 antibody

• Stress 70 protein mitochondrial antibody

• Stress 70 protein mitochondrial precursor antibody

• Stress-70 protein antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)

  Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Grp75/MOT (green) with ab2799. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA for 15 minutes at room temperature. Cells were incubated with (left panel) or without (right panel) ab2799 (1:50) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody (1:400) for 30 minutes at room temperature. F-Actin (red) was stained with Dylight 554 phalloidin, and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody [JG1] (ab2799)

  Immunohistochemistry was performed on normal biopsies of deparaffinized human testis tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a Mouse Monoclonal Antibody recognizing Grp75/MOT (ab2799) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

• 

  Flow Cytometry - Anti-Grp75/MOT antibody [JG1] (ab2799)

  Overlay histogram showing HepG2 cells stained with ab2799 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2799, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 [MG3-35] (ab18394, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)

  ICC/IF image of ab2799 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2799, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)

  Immunocytochemistry/ Immunofluorescence of P19 (Mouse embryonal carcinoma cell line) cells using ab2799.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)

  Immunocytochemistry/ Immunofluorescence of NS-1 (Mouse myeloma cell line) cells using ab2799.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)

  Immunocytochemistry/ Immunofluorescence of HMVEC (Human microvascular endothelial cell line) cells using ab2799.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)

  Immunocytochemistry/ Immunofluorescence of A549 (Human lung carcinoma cell line) cells using ab2799.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody [JG1] (ab2799)

  ab2799 (1µg/ml) staining GrP75/MOT in human frontal cerebral cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is strong mitochondrial staining.
  Insert depicts negative control (no primary antibody).
  Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (ab2799)

  Immunocytochemistry/Immunofluorescence analysis of human fibroblasts labelling Grp75/MOT with ab2799.