Anti-Clathrin heavy chain antibody [X22]

• 产品名称

  Anti-Clathrin heavy chain抗体[X22]
  参阅全部 Clathrin heavy chain 一抗

• 描述

  小鼠单克隆抗体[X22] to Clathrin heavy chain

• 宿主

  Mouse

• 经测试应用

  适用于: WB, IHC-P, ICC/IFmore details

• 种属反应性

  与反应: Human, Xenopus laevis
  

• 免疫原

  Full length native protein (purified) corresponding to Human Clathrin heavy chain. Purified human brain clathrin heavy chain.

• 表位

  Electron microscopy and proteolysis mapping demonstrate that binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituent: PBS

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  X22

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2731于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 1213 Human

• Omim: 118955 Human

• SwissProt: P53675 Human

• SwissProt: Q00610 Human

• Unigene: 491351 Human

• 别名

• Clathrin heavy chain 2 antibody

• Clathrin heavy chain like 1 antibody

• Clathrin heavy chain on chromosome 17 antibody

• Clathrin heavy polypeptide antibody

• CLH 17 antibody

• CLH 22 antibody

• CLH-17 antibody

• CLH1_HUMAN antibody

• CLH17 antibody

• CLH22 antibody

• CLTC antibody

• CLTCL antibody

• CLTCL1 antibody

• CLTCL2 antibody

• CLTD antibody

• Hc antibody

• KIAA0034 antibody

• CHC17 antibody

• Clathrin heavy chain antibody

• Clathrin heavy chain 1 antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [X22] (ab2731)

  Immunocytochemistry/Immunofluorescence analysis of Clathrin heavy chain shows staining in HeLa cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2731 (1:200) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.

• 

  Western blot - Anti-Clathrin heavy chain antibody [X22] (ab2731)

  Anti-Clathrin heavy chain antibody [X22] (ab2731) at 1/300 dilution + Human brain lysates at 25 µg
  
  Secondary
  HRP-conjugated goat anti-mouse IgG + IgM (H+L)
  
  Developed using the ECL technique.
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [X22] (ab2731)

  Immunohistochemistry was performed on normal biopsies of deparaffinized Human colon tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Clathrin Heavy chain ab2731 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [X22] (ab2731)

  Immunocytochemistry/Immunofluorescence analysis of Clathrin heavy chain shows staining in NCI-H460 cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2731 (1:200) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [X22] (ab2731)

  Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Clathrin Heavy chain ab2731 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [X22] (ab2731)

  Immunocytochemistry/Immunofluorescence analysis of Clathrin heavy chain shows staining in U251 cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2731 (1:200) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [X22] (ab2731)

  Xenopus laevis cytoplasmic egg extract visualized live with primary and secondary antibody addition [red is anti-Clathrin heavy chain X22 (ab2731) with goat anti-mouse Alexa Fluor 568 secondary, green is anti-HIP1R (ab77297) with goat anti-rabbit Alexa Fluor 488 secondary]. Large red structures are probably aggregates, but the small structures appear to be specific for vesicle staining.

  See Abreview

• 

  Western blot - Anti-Clathrin heavy chain antibody [X22] (ab2731)

  All lanes : Anti-Clathrin heavy chain antibody [X22] (ab2731) at 1/500 dilution
  
  Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Observed band size: 180 kDawhy is the actual band size different from the predicted?
  Additional bands at: 240 kDa, 450 kDa. We are unsure as to the identity of these extra bands.