Goat F(ab')2 Anti-Rat IgG - Fc (HRP), pre-adsorbed

• 产品名称

  山羊F(ab')2抗大鼠IgG - Fc (HRP),预吸附二抗
  参阅全部 IgG 二抗

• 宿主

  Goat

• 靶标种属

  Rat

• 特异性

  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab) or Bovine, Horse, Human Serum Proteins.

• 经测试应用

  适用于: ICC/IF, Dot blot, WB, ELISA, IHC, Electron Microscopymore details

• 最小交叉反应

  
  Cow, Horse, Humanmore details

• 免疫原

  Rat IgG F(c) whole molecule

• 偶联物

  HRP

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C.

• 存储溶液

  Preservative: 0.01% Gentamicin sulphate
  Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
  
  BSA is IgG and protease free. Warning: use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

• 浓度

• 500 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 纯化说明

  This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

• 共轭说明

  Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab6257于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Dot: Use at an assay dependent dilution.
Electron Microscopy: Use at an assay dependent dilution.
ELISA: 1/20000 - 1/200000.
IHC: 1/500 - 1/5000.
WB: 1/20000 - 1/200000.

This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20000 to 1/200000 of the reconstitution concentration is suggested for this product for ELISA and Western blots and a 1/500 to 1/5000 for immunohistochemistry on tissue sections.

Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.