Anti-Progesterone Receptor antibody [Alpha PR6]

• 产品名称

  Anti-Progesterone Receptor抗体[Alpha PR6]
  参阅全部 Progesterone Receptor 一抗

• 描述

  小鼠单克隆抗体[Alpha PR6] to Progesterone Receptor

• 宿主

  Mouse

• 特异性

  Detects the B form of the progesterone receptor (PR). This antibody does not cross-react with estrogen receptor or glucocorticoid receptor.

• 经测试应用

  适用于: IHC-P, WB, Flow Cyt, ICC/IFmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Full length protein corresponding to Chicken Progesterone Receptor. Progesterone receptor purified from chick oviduct cytosol.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituent: PBS

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  Alpha PR6

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab2765于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 5241 Human

• Omim: 607311 Human

• SwissProt: P06401 Human

• Unigene: 32405 Human

• Unigene: 742403 Human

• 别名

• PR antibody

• PRA antibody

• PRB antibody

• PRGR_HUMAN antibody

• Progesterone receptor antibody

• Progestin receptor form A antibody

• Progestin receptor form B antibody

• NR3C3 antibody

• Nuclear receptor subfamily 3 group C member 3 antibody

• PGR antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [Alpha PR6] (ab2765)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on human uterus tissue. Antigen retrieval was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2765 (1:20) overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)

  Immunocytochemistry/ Immunofluorescence analysis of T47D cells untreated (left) or stimulated with 100nm promegestone for 1 hour (right), labeling Progesterone Receptor with ab2765 (green). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA for 15 minutes at room temperature. Cells were stained with Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765) at a dilution of 1/100 for 1 hour at 37C, and then incubated with a Alexa Fluor 488 goat anti-mouse IgG secondary antibody at a dilution of 1/1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye.

• 

  Western blot - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)

  All lanes : Anti-Progesterone Receptor antibody [Alpha PR6] (ab2765) at 1 µg/ml
  
  Lane 1 : T47D cell lysate untreated (-)
  Lane 2 : T47D cell lysate stimulated (+) with 100 nm promegestone for 1 hour
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat anti-Mouse IgG-HRP at 1/2000 dilution
  
  Predicted band size: 99 kDa
  
  

• 

  Flow Cytometry - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)

  Overlay histogram showing T47D cells stained with ab2765 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2765, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.