Anti-Hsp70 antibody [3A3]

• 产品名称

  Anti-Hsp70抗体[3A3]
  参阅全部 Hsp70 一抗

• 描述

  小鼠单克隆抗体[3A3] to Hsp70

• 宿主

  Mouse

• 特异性

  This antibody detects several members of the heat shock protein 70 kDa (Hsp 70) gene family including Hsp 70, Hsc 70, p75, and following heat shock, Hsp 72 from yeast, Drosophila, fish, mouse, avian, amphibian and human samples.

• 经测试应用

  适用于: ICC/IF, IHC-P, WBmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Recombinant fragment corresponding to Human Hsp70 aa 504-617 (C terminal).

• 表位

  Epitope mapping with a panel of Hsp 70 deletion mutants suggests that the epitope recognized is located between amino acids 504-617 of human Hsp 70, a region that has been shown to be involved in stress-induced nucleolar localization.

• 阳性对照

• WB: HeLa, K562, Jurkat, 293T, A431, A549, F9, RAW264.7 and C6, PC-12 lysates. IHC-P: Human cerebrum and lung carcinoma, Mouse testis, Rat cerebrum tissues. ICC/IF: HeLa and NIH/3T3, PC-12 cells.

• 常规说明

  This product has switched from a hybridoma to recombinant production method on 08th March 2021.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

• 浓度

• 10 µg 浓度为 1.053 mg/ml

• 批次浓度范围 100 µg 浓度为 1.044 - 1.068 mg/ml

• 纯度

  Protein A purified

• Primary antibody说明

  The Hsp 70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human Hsp 70 family members include: Hsp 70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; Hsp 72, a 72 kDa protein that is induced exclusively under stress conditions; Hsc 70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or Hsp 75, a 75 kDa protein that is found within the mitochondria.

• 克隆

  单克隆

• 克隆编号

  3A3

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab5439于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 15511 Human

• Entrez Gene: 3303 Human

• Entrez Gene: 193740 Mouse

• Entrez Gene: 3304 Mouse

• Entrez Gene: 24472 Rat

• Entrez Gene: 294254 Rat

• Omim: 140550 Human

• Omim: 603012 Human

• SwissProt: P0DMV8 Human

• SwissProt: P0DMV9 Human

• SwissProt: P17879 Mouse

• SwissProt: Q61696 Mouse

• SwissProt: P0DMW0 Rat

• SwissProt: P0DMW1 Rat

别名

• DnaK type molecular chaperone HSP70 1 antibody

• Epididymis secretory protein Li 103 antibody

• FLJ54303 antibody

• FLJ54370 antibody

• FLJ54392 antibody

• FLJ54408 antibody

• FLJ75127 antibody

• Heat shock 70 kDa protein 1 antibody

• Heat shock 70 kDa protein 1/2 antibody

• Heat shock 70 kDa protein 1A antibody

• Heat shock 70 kDa protein 1A/1B antibody

• Heat shock 70kDa protein 1B antibody

• Heat shock induced protein antibody

• HEL S 103 antibody

• HSP70 1 antibody

• HSP70 1A antibody

• HSP70 1B antibody

• HSP70 2 antibody

• HSP70-1 antibody

• HSP70-1/HSP70-2 antibody

• HSP70-1A antibody

• HSP70-2 antibody

• HSP70.1 antibody

• HSP70.1/HSP70.2 antibody

• HSP71_HUMAN antibody

• HSP72 antibody

• HSPA1 antibody

• HSPA1A antibody

• HSPA1B antibody

• 

  Western blot - Anti-Hsp70 antibody [3A3] (ab5439)

  All lanes : Anti-Hsp70 antibody [3A3] (ab5439) at 1/1000 dilution
  
  Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
  Lane 2 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
  Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
  Lane 4 : 293T (human embryonic kidney epithelial cell), whole cell lysate
  Lane 5 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
  Lane 6 : A549 (human lung carcinoma epithelial cell), whole cell lysate
  Lane 7 : F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate
  Lane 8 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
  Lane 9 : C6 (rat glial tumor glial cell), whole cell lysate
  Lane 10 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
  
  Predicted band size: 70-78 kDa
  Observed band size: 70 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 1 second
  
  
  

  Blocking/Diluting buffer and concentration: 5% NFDM/TBST

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [3A3] (ab5439)

  Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Hsp70 with ab5439 at 1/2000 (0.519 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with ab5439 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [3A3] (ab5439)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling Hsp70 with ab5439 at 1/50 (20.76 µg/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in HeLa cells ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [3A3] (ab5439)

  Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Hsp70 with ab5439 at 1/2000 (0.519 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human lung carcinoma. The section was incubated with ab5439 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [3A3] (ab5439)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Hsp70 with ab5439 at 1/50 (20.76 µg/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in NIH/3T3 cells ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [3A3] (ab5439)

  Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Hsp70 with ab5439 at 1/2000 (0.519 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse testis. The section was incubated with ab5439 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [3A3] (ab5439)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 cells labelling Hsp70 with ab5439 at 1/50 (20.76 µg/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cells ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [3A3] (ab5439)

  Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Hsp70 with ab5439 at 1/2000 (0.519 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab5439 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Anti-Hsp70 antibody [3A3] (ab5439)