Anti-nNOS (neuronal) (phospho S1417)抗体
参阅全部 nNOS (neuronal) 一抗
兔多克隆抗体to nNOS (neuronal) (phospho S1417)
Rabbit
适用于: IHC-Fr, ICC, WB, IP, ICC/IF, IHC-FoFrmore details
与反应: Mouse, Rat, Rabbit, Human
预测可用于: Xenopus laevis, Zebrafish
Synthetic peptide corresponding to Human nNOS (neuronal) aa 1411-1425 (phospho S1417).
Sequence:
CNRLRSE(pS)IAFIEESK
WB: nocozadole-treated SH-SY5Y cells; ICC: SH-SY5Y cells
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab5583于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | (2) | Use at an assay dependent concentration. |
| ICC | Use at an assay dependent concentration. | |
| WB | (4) | 1/500 - 1/5000. Predicted molecular weight: 160 kDa. |
| IP | Use at an assay dependent concentration. | |
| ICC/IF | 1/10 - 1/100. | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 20881244 |
Entrez Gene: 4842 Human
Entrez Gene: 18125 Mouse
Entrez Gene: 100009243 Rabbit
Entrez Gene: 60658 Zebrafish
Omim: 163731 Human
SwissProt: P29475 Human
SwissProt: Q9Z0J4 Mouse
SwissProt: O19132 Rabbit
Unigene: 654410 Human
Unigene: 684465 Human
Unigene: 684466 Human
Unigene: 684467 Human
Unigene: 442195 Mouse
Unigene: 44249 Mouse
Unigene: 10573 Rat
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BNOS antibody
Constitutive NOS antibody
EC 1.14.13.39 antibody
IHPS 1 antibody
IHPS1 antibody
N-NOS antibody
NC-NOS antibody
neuronal Nitric Oxide Synthase antibody
Neuronal NOS antibody
Nitric oxide synthase , neuronal, included antibody
Nitric oxide synthase 1 (neuronal) antibody
Nitric oxide synthase 1 antibody
Nitric oxide synthase, brain antibody
Nitric oxide synthase, penile neuronal, included antibody
NNOS antibody
NO antibody
NOS 1 antibody
NOS antibody
NOS type I antibody
NOS-I antibody
NOS1 antibody
NOS1_HUMAN antibody
Peptidyl-cysteine S-nitrosylase NOS1 antibody
Western blot - Anti-nNOS (neuronal) (phospho S1417) antibody (ab5583)
All lanes : Anti-nNOS (neuronal) (phospho S1417) antibody (ab5583) at 1/1000 dilution
Lane 1 : untreated SH-SY5Y cells (Human neuroblastoma cell line from bone marrow)
Lane 2 : SH-SY5Y cells (Human neuroblastoma cell line from bone marrow) treated with 100 ng/mL nocodazole
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated secondary antibody
Developed using the ECL technique.
Predicted band size: 160 kDa
Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) (phospho S1417) antibody (ab5583)
Immunocytochemistry/Immunofluorescence analysis of nNOS (neuronal) (phospho S1417) (green) showing staining in the cytoplasm and nucleus of SH-SY5Y cells treated with 100 ng/ml nocodazole (left) and untreated SH-SY5Y cells (right). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5583 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
