兔F(ab')2抗山羊IgG - H&L (Biotin),预吸附二抗
参阅全部 IgG 二抗
Rabbit
Goat
No reaction was observed against Human Serum Proteins.
适用于: WB, ICC/IF, Dot blot, ELISA, IHC, Immunomicroscopy, Flow Cytmore details
Humanmore details
Goat IgG whole molecule
Biotin
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
BSA is IgG and protease free
浓度
500 µg 浓度为 1 mg/ml
Affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
多克隆
IgG
Abpromise™承诺保证使用ab5753于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/2000 - 1/10000. | |
ICC/IF | 1/1000 - 1/5000. | |
Dot blot | 1/10000. | |
ELISA | 1/20000 - 1/100000. | |
IHC | 1/500 - 1/5000. | |
Immunomicroscopy | Use at an assay dependent dilution. | |
Flow Cyt | 1/200 - 1/1000. |