Anti-Rhodopsin antibody [1D4]

• 产品名称

  Anti-Rhodopsin抗体[1D4]
  参阅全部 Rhodopsin 一抗

• 描述

  小鼠单克隆抗体[1D4] to Rhodopsin

• 宿主

  Mouse

• 特异性

  ab5417 detects Rhodopsin from human and bovine retinal samples. Data from Yin J et al., 2012 (PMID 22743318) indicates that in Zebrafish ab5417 appears to recognize Red Opsin rather than Rhodopsin.

• 经测试应用

  适用于: ELISA, IHC-FoFr, IP, WB, IHC-Fr, IHC-P, ICC/IFmore details

• 种属反应性

  与反应: Mouse, Rat, Cow, Human, Zebrafish, Amphibian
  预测可用于: Rabbit

• 免疫原

  Tissue, cells or virus corresponding to Bovine Rhodopsin. Bleached bovine ROS [rod outer segment] disk membranes

• 表位

  The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope.

• 阳性对照

• WB: HL60 whole cell lysate IHC-P: Human and mouse retinal tissue

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 99% PBS, 0.1% BSA

• 浓度

• 100 µl 浓度为 1 mg/ml

• 纯度

  Protein G purified

• Primary antibody说明

  Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin’s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin.

• 克隆

  单克隆

• 克隆编号

  1D4

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab5417于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 509933 Cow

• Entrez Gene: 100307115 Human

• Entrez Gene: 6010 Human

• Entrez Gene: 212541 Mouse

• Entrez Gene: 100009200 Rabbit

• Entrez Gene: 24717 Rat

• Entrez Gene: 30295 Zebrafish

• Omim: 180380 Human

• SwissProt: P02699 Cow

• SwissProt: P08100 Human

• SwissProt: P15409 Mouse

• SwissProt: P49912 Rabbit

• SwissProt: P51489 Rat

• SwissProt: P35359 Zebrafish

• Unigene: 247565 Human

• Unigene: 2965 Mouse

• Unigene: 406156 Mouse

• Unigene: 92530 Rat

• Unigene: 354 Zebrafish

别名

• CSNBAD1 antibody

• MGC138309 antibody

• MGC138311 antibody

• OPN 2 antibody

• OPN2 antibody

• opsd antibody

• OPSD_HUMAN antibody

• opsin 2 antibody

• Opsin 2 rod pigment antibody

• Opsin-2 antibody

• Opsin2 antibody

• Retinitis Pigmentosa 4 antibody

• Retinitis pigmentosa 4 autosomal dominant antibody

• RHO antibody

• Rhodopsin antibody

• RP 4 antibody

• RP4 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)Pearring et al PLoS Genet. 2017 Apr 14;13(4):e1006740. doi: 10.1371/journal.pgen.1006740. eCollection 2017 Apr. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  Deletion of Arf4 mice from the retina does not disrupt rhodopsin localization or photoreceptor morphology.

  E. Arf4 immunostaining in Arf4flox/CagCreER experimental and control retinal cross-sections. Image of the photoreceptor IS where the biosynthetic membranes are localized. Eyes were collected at P34. Scale bar = 10 μm.

  F. Rhodopsin immunostaining in Arf4flox/CagCreER experimental and control retinal cross-sections. Eyes were collected at P34. Scale bar = 10 μm.

  G. Comparative analysis of photoreceptor morphology in Arf4flox/CagCreER experimental and control retinal cross-sections. Eyes were collected at P41. Scale bar = 20 μm.

  OS = outer segment, IS = inner segment, ONL = outer nuclear layer, OPL = outer plexiform layer.

• 

  Western blot - Anti-Rhodopsin antibody [1D4] (ab5417)

  Anti-Rhodopsin antibody [1D4] (ab5417) at 1/500 dilution + HL60 (Human promyelocytic leukemia cell line) cell lysate at 25 µg
  
  Observed band size: 40 kDawhy is the actual band size different from the predicted?
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)

  Immunohistochemical analysis of formalin-fixed mouse retinal tissue, labeling rhodopsin with ab5417 at a 1:50 dilution in 3% BSA-PBS solution and incubated at 4°C overnight in a high humidity environment.

  A DyLight® 488 secondary antibody was used (green) incubated at room temperature in the dark. The tissue was counterstained with DAPI against DNA, showing nuclear compartments. Prior to staining the formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, then blocked with 3% BSA-PBS for 30 minutes at room temperature.

  The left image is a negative control with only the secondary antibody and the right image is in the presence of ab5417 and the secondary.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)

  Immunohistochemical analysis of formalin-fixed human retinal tissue, labeling rhodopsin with ab5417 at a 1:50 dilution in 3% BSA-PBS solution and incubated at 4°C overnight in a high humidity environment.

  A DyLight® 488 secondary antibody was used (green) incubated at room temperature in the dark. The tissue was counterstained with DAPI against DNA, showing nuclear compartments. Prior to staining the formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, then blocked with 3% BSA-PBS for 30 minutes at room temperature.

  The left image is a negative control with only the secondary antibody and the right image is in the presence of ab5417 and the secondary.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)

  Immunohistochemical analysis of paraffin-embedded mouse retinal tissue labeling Rhodopsin with ab5417.

  Secondary used was HRP conjugated. Prior preparation was initiated by antigen retrival using 10mM sodium citrate at pH 6.0, then the sample was microwaved for 8 to 15 minutes. Subsequent to retrival the retinal tissue was blocked for 15 minutes at room temperature with 3% hydrogen peroxide. The sample was then incubated with ab5417 in 3% BSA-PBS at 4°C at a dilution of 1:1000, overnight. Hematoxylin was used to counterstain the tissue.

  The left side of the image is shown as a negative control and is the tissue in the absence of ab5417, the right side is in the prescence of the counterstain, ab5417 and the HRP conjugated secondary.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)

  Immunohistochemical analysis of paraffin-embedded human retinal tissue labeling Rhodopsin with ab5417.

  Secondary used was HRP conjugated. Prior preparation was initiated by antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Subsequent to retrival the retinal tissue was blocked in 3% H2O2-methanol for 15 min at room temperature. The sample was then incubated with ab5417 in 3% BSA-PBS at a dilution of 1:200 overnight at 4°C, overnight. Hematoxylin was used to counterstain the tissue.

  The left side of the image is shown as a negative control and is the tissue in the absence of ab5417, the right side is in the prescence of the counterstain, ab5417 and the HRP conjugated secondary.