Anti-nNOS (neuronal)抗体
参阅全部 nNOS (neuronal) 一抗
兔多克隆抗体to nNOS (neuronal)
Rabbit
适用于: ICC/IF, WB, IHC-Pmore details
与反应: Mouse, Rat, Human
预测可用于: Rabbit, Xenopus laevis, Zebrafish
Synthetic peptide corresponding to Human nNOS (neuronal) aa 1411-1425.
Sequence:
NRLRSESIAFIEESK
(Peptide available as ab5891)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab5586于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/50 - 1/500. | |
| WB | 1/100 - 1/1000. Predicted molecular weight: 160 kDa. | |
| IHC-P | (1) | 1/20 - 1/200. |
Entrez Gene: 4842 Human
Entrez Gene: 18125 Mouse
Entrez Gene: 100009243 Rabbit
Entrez Gene: 60658 Zebrafish
Omim: 163731 Human
SwissProt: P29475 Human
SwissProt: Q9Z0J4 Mouse
SwissProt: O19132 Rabbit
Unigene: 654410 Human
Unigene: 684465 Human
Unigene: 684466 Human
Unigene: 684467 Human
Unigene: 442195 Mouse
Unigene: 44249 Mouse
Unigene: 10573 Rat
2310005C01Rik antibody
BNOS antibody
Constitutive NOS antibody
EC 1.14.13.39 antibody
IHPS 1 antibody
IHPS1 antibody
N-NOS antibody
NC-NOS antibody
neuronal Nitric Oxide Synthase antibody
Neuronal NOS antibody
Nitric oxide synthase , neuronal, included antibody
Nitric oxide synthase 1 (neuronal) antibody
Nitric oxide synthase 1 antibody
Nitric oxide synthase, brain antibody
Nitric oxide synthase, penile neuronal, included antibody
NNOS antibody
NO antibody
NOS 1 antibody
NOS antibody
NOS type I antibody
NOS-I antibody
NOS1 antibody
NOS1_HUMAN antibody
Peptidyl-cysteine S-nitrosylase NOS1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)
ab5586 labelling nNOS (neuronal) in the cytoplasm of Mouse heart tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (ab5586)
Immunocytochemistry/Immunofluorescence analysis of nNOS (neuronal) (green) showing staining in the cytoplasm and membrane of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5586 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)
ab5586 labelling nNOS (neuronal) in the cytoplasm of Human testis tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)
ab5586 labelling nNOS (neuronal) in the cytoplasm of Human skeletal muscle (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Western blot - Anti-nNOS (neuronal) antibody (ab5586)
Anti-nNOS (neuronal) antibody (ab5586) at 1/500 dilution + Mouse brain cell lysate at 25 µg
Predicted band size: 160 kDa
Observed band size: 160 kDa
Western blot - Anti-nNOS (neuronal) antibody (ab5586)
Anti-nNOS (neuronal) antibody (ab5586) at 1/200 dilution + Rat brain cell lysate at 25 µg
Predicted band size: 160 kDa
Observed band size: 160 kDa
