Anti-Met (c-Met) (phospho Y1230 + Y1234 + Y1235)抗体
参阅全部 Met (c-Met) 一抗
兔多克隆抗体to Met (c-Met) (phospho Y1230 + Y1234 + Y1235)
Rabbit
The phosphospecific antibody that has been generated does not distinguish between the dually (pYpY 1234/1235) and triply (pYpYpY1230/1234/1235) phosphorylated forms of c-Met, both of which are likely to represent activated forms of this receptor.
适用于: WBmore details
与反应: Human
预测可用于: Mouse, Rat
Synthetic peptide corresponding to Met (c-Met) (phospho Y1230 + Y1234 + Y1235).
WB: HEK-293T whole cell lysate.
Binding of scatter factor (SF)/hepatocyte growth factor (HGF) to the c Met receptor tyrosine kinase (RTK) triggers receptor dimerization and phosphorylation on multiple residues within the juxtamembrane, catalytic core and cytoplasmic tail domains, thereby regulating receptor internalization, catalytic activity and multisubstrate docking. c Met contains three tyrosines (Tyr-xx- x-Tyr-Tyr motif) within the activation loop of the catalytic domain. This is also seen with the insulin receptor, insulin-like growth factor receptor (IGF1) receptor and nerve growth factor (NGF) receptors/Trks, for which phosphorylation of all three tyrosines is required for full activation. With c Met (and the related family member, RON) phosphorylation of tyrosines 1234 and 1235 has been shown to be important in receptor activation. Activation of the c Met receptor results in binding and/or phosphorylation of many intracellular signaling proteins including multiple adaptor proteins (e.g., Grb2, Shc, Cbl, Crk, cortactin, paxillin, and GAB1), and a variety of other signal transducers (e.g., PI 3-kinase, FAK, Src, Erk1&2, JNK, PLC-ã, and STAT3).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
浓度
50 µl 浓度为 0.4 mg/ml
Immunogen affinity purified
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated c-Met protein. The final product is generated by affinity chromatography using a c Met-derived peptide that is phosphorylated at tyrosines 1230, 1234, 1235.
Binding of scatter factor (SF)/hepatocyte growth factor (HGF) to the c Met receptor tyrosine kinase (RTK) triggers receptor dimerization and phosphorylation on multiple residues within the juxtamembrane, catalytic core and cytoplasmic tail domains, thereby regulating receptor internalization, catalytic activity and multisubstrate docking. c Met contains three tyrosines (Tyr-xx- x-Tyr-Tyr motif) within the activation loop of the catalytic domain. This is also seen with the insulin receptor, insulin-like growth factor receptor (IGF1) receptor and nerve growth factor (NGF) receptors/Trks, for which phosphorylation of all three tyrosines is required for full activation. With c Met (and the related family member, RON) phosphorylation of tyrosines 1234 and 1235 has been shown to be important in receptor activation. Activation of the c Met receptor results in binding and/or phosphorylation of many intracellular signaling proteins including multiple adaptor proteins (e.g., Grb2, Shc, Cbl, Crk, cortactin, paxillin, and GAB1), and a variety of other signal transducers (e.g., PI 3-kinase, FAK, Src, Erk1&2, JNK, PLC-ã, and STAT3).
多克隆
IgG
Abpromise™承诺保证使用ab5662于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) | 1/1000. Detects a band of approximately 169 kDa. |
Entrez Gene: 4233 Human
Entrez Gene: 17295 Mouse
Omim: 164860 Human
SwissProt: P08581 Human
SwissProt: P16056 Mouse
Unigene: 132966 Human
Unigene: 86844 Mouse
Unigene: 10617 Rat
AUTS9 antibody
c met antibody
D249 antibody
Hepatocyte growth factor receptor antibody
HGF antibody
HGF receptor antibody
HGF/SF receptor antibody
HGFR antibody
MET antibody
Met proto oncogene antibody
Met proto oncogene tyrosine kinase antibody
MET proto oncogene, receptor tyrosine kinase antibody
Met proto-oncogene (hepatocyte growth factor receptor) antibody
Met proto-oncogene antibody
Met protooncogene antibody
MET_HUMAN antibody
Oncogene MET antibody
Par4 antibody
Proto-oncogene c-Met antibody
RCCP2 antibody
Scatter factor receptor antibody
SF receptor antibody
Tyrosine-protein kinase Met antibody
Western blot - Anti-Met (c-Met) (phospho Y1230 + Y1234 + Y1235) antibody (ab5662)
All lanes : Anti-Met (c-Met) (phospho Y1230 + Y1234 + Y1235) antibody (ab5662) at 1/1000 dilution
Lane 1 : Unstimulated (-), HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lanes 2-5 : Stimulated (+) with HGF, HEK-293T whole cell extract
Secondary
All lanes : Goat F(ab’)2 antirabbit IgG HRP conjugate
Peptide Competition:
Prior primary antibody incubation:
1 and 2 - no peptide,
3 - non-phosphopeptide corresponding to the immunogen,
4 -generic phosphotyrosine-containing peptide),
5 - phosphopeptide immunogen.
SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C.
Bands were detected using the Pierce SuperSignal method.
The data show that only the phosphopeptide corresponding to c Met pYpYpY1230/1234/1235] block the antibody signal, demonstrating the specificity of the antibody.
Note: There are three isoforms of c Met, two of which are recognized by this antibody.
