Anti-FANCC抗体
参阅全部 FANCC 一抗
兔多克隆抗体to FANCC
Rabbit
Detects a band at 60kDa in Hela cell lysate corresponding to FANCC.
适用于: ICC/IF, WBmore details
与反应: Human
不与反应: Mouse
Synthetic peptide:
CLINKEPQNSGSKLNS
, corresponding to amino acids 96 - 112 of Human FANCC.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
Immunogen affinity purified
This affinity-purified antibody is directed against human FANCC protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
多克隆
IgG
Abpromise™承诺保证使用ab5065于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | (6) | 1/1000 - 1/3000. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa). |
Entrez Gene: 2176 Human
Omim: 613899 Human
SwissProt: Q00597 Human
Unigene: 494529 Human
FAC antibody
FACC antibody
FANCC antibody
FANCC_HUMAN antibody
Fanconi anemia complementation group C antibody
Fanconi anemia complementation group C protein antibody
Fanconi anemia group C protein antibody
Fanconi pancytopenia type 3 antibody
FLJ14675 antibody
Protein FACC antibody
bA80I15.1 antibody
FA 3 antibody
FA3 antibody
Western blot - Anti-FANCC antibody (ab5065)
Anti-FANCC antibody (ab5065) at 1/1500 dilution + HeLa whole cell lysate at 35 µg
Secondary
IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size: 63 kDa
Observed band size: 60 kDawhy is the actual band size different from the predicted?
Additional bands at: 33 kDa. We are unsure as to the identity of these extra bands.
Primary antibody incubated with PBS supplemented with 1% normal goat serum and 0.1% milk.
Immunocytochemistry/ Immunofluorescence - Anti-FANCC antibody (ab5065)
ICC/IF image of ab5065 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5065, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
