Anti-LXR alpha antibody

• 产品名称

  Anti-LXR alpha抗体
  参阅全部 LXR alpha 一抗

• 描述

  兔多克隆抗体to LXR alpha

• 宿主

  Rabbit

• 特异性

  Detects recombinant Human LXR alpha. This antibody does not detect recombinant Human LXR beta.

• 经测试应用

  适用于: ICC, IHC-FoFr, ICC/IFmore details
  不适用于: WB

• 种属反应性

  与反应: Mouse, Rat, Human, Chinese hamster
  预测可用于: Cow

• 免疫原

  Synthetic peptide corresponding to Human LXR alpha aa 318-329.
  Sequence:

  DFSYNREDFAKA

  
  (Peptide available as ab5021)

  Run BLAST with Run BLAST with 

• 阳性对照

• ICC: MCF7, HeLa cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 99% PBS, 0.1% BSA

• 浓度

• 200 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• Primary antibody说明

  Liver X Receptors (LXRs) are oxysterol activated nuclear receptors which are involved in the regulation of genesis and metabolism of cholesterols and bile acids. LXRs form heterodimeric complexes with the retinoic acid receptors (RARs) once activated by their respective oxysterol ligands. Two known isoforms, alpha and beta, are differentially expressed; the alpha isoform predominantly expressed in liver, whereas beta isoform expression has been shown to be ubiquitous.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab3585于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for WB.

数据库链接

• Entrez Gene: 507176 Cow

• Entrez Gene: 10062 Human

• Entrez Gene: 22259 Mouse

• Entrez Gene: 58852 Rat

• Omim: 602423 Human

• SwissProt: Q5E9B6 Cow

• SwissProt: Q13133 Human

• SwissProt: Q9Z0Y9 Mouse

• SwissProt: Q62685 Rat

• Unigene: 438863 Human

• Unigene: 22690 Mouse

• Unigene: 11209 Rat

别名

• Liver X receptor alpha antibody

• LXR a antibody

• LXRA antibody

• NR1H3 antibody

• NR1H3_HUMAN antibody

• Nuclear receptor subfamily 1 group H member 3 antibody

• Oxysterols receptor LXR alpha antibody

• Oxysterols receptor LXR-alpha antibody

• RLD 1 antibody

• RLD1 antibody

• 

  Immunocytochemistry - Anti-LXR alpha antibody (ab3585)

  Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.

• 

  Immunocytochemistry - Anti-LXR alpha antibody (ab3585)

  Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.