Anti-ARIH1 antibody

• 产品名称

  Anti-ARIH1抗体

• 描述

  山羊多克隆抗体to ARIH1

• 宿主

  Goat

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide:

  HEGYEKDLWEYIED

  , corresponding to C terminal amino acids 544-557 of Human ARIH1.

  Run BLAST with Run BLAST with 

• 阳性对照

• U937 lysate .

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 0.5% Tris, 0.5% BSA

• 纯度

  Immunogen affinity purified

• 纯化说明

  Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab3891于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Western Blot: Approx 65 kDa band seen in U937 lysate [Predicted MW of approx. 61kDa according to NP_005735].
Recommended for use at 0.2-1.0µg/ml.
Not tested in other applications.

Optimal working dilutions / concentrations should be determined by the end user.

• 数据库链接

• Entrez Gene: 25820 Human

• Omim: 605624 Human

• SwissProt: Q9Y4X5 Human

• Unigene: 268787 Human

• 别名

• ARI1 antibody

• ARI1_HUMAN antibody

• Ariadne Drosophila homolog of antibody

• Ariadne homolog antibody

• Ariadne homolog ubiquitin conjugating enzyme E2 binding protein 1 antibody

• Ariadne ubiquitin conjugating enzyme E2 binding protein homolog 1 antibody

• ARIH 1 antibody

• ARIH1 antibody

• E3 ubiquitin protein ligase ARIH1 antibody

• H7 AP2 antibody

• H7-AP2 antibody

• HARI antibody

• HHARI antibody

• human homologue of ariadne antibody

• Monocyte protein 6 antibody

• MOP 6 antibody

• MOP-6 antibody

• MOP6 antibody

• Protein ariadne 1 homolog antibody

• Protein ariadne-1 homolog antibody

• Protein ariadne1 homolog antibody

• UbcH7 binding protein antibody

• UBCH7 BP antibody

• UbcH7-binding protein antibody

• UBCH7BP antibody

• UbcM4 interacting protein antibody

• UbcM4-interacting protein antibody

• Ubiquitin conjugating enzyme E2 binding protein 1 antibody

• Ubiquitin-conjugating enzyme E2-binding protein 1 antibody

• ARI 1 antibody

• ARI antibody

• ARI-1 antibody

• 

  Western blot - Anti-ARIH1 antibody (ab3891)This image is courtesy of an Abreview submitted by T Lawson

  All lanes : Anti-ARIH1 antibody (ab3891) at 1/1500 dilution
  
  Lanes 1-2 : NIH3T3 whole cell lysate at 30 µg
  Lane 3 : Partially purified NIH3T3 protein mixture at 10 µg
  
  Secondary
  All lanes : Alkaline Phosphatase-conjugated anti-goat IgG polyclonal at 1/15000 dilution
  
  Performed under reducing conditions.
  
  Observed band size: 65 kDawhy is the actual band size different from the predicted?
  Additional bands at: 45 kDa (possible non-specific binding), 50 kDa (possible non-specific binding)
  
  
  Exposure time: 15 minutes
  
  
  

  Lanes 1 and 2 - 12% SDS-PAGE.

  Lane 3 - 4-15% SDS-PAGE.

  Lanes 1 and 3 - developed with colorimetric substrate.

  Lane 2 - developed with ECF+.

  See Abreview

• 

  Western blot - Anti-ARIH1 antibody (ab3891)

  ab3891 staining (0.5µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab3891 staining (0.5µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.