Anti-Caspase-3抗体
参阅全部 Caspase-3 一抗
兔多克隆抗体to Caspase-3
Rabbit
Caspase-3 is a member of the interleukin-1 β-converting enzyme family. Caspase-3 is thought to be associated with induction of apoptosis. Caspase-3 is synthesized as inactive 32 kDa proenzyme and is processed during apoptosis generating two subunits of 17 kDa and 12 kDa. Caspase-3 stains the epithelial cells of skin, renal proximal tubules and collecting ducts.
This antibody reacts with the 32 kDa proenzyme and the 17 kDa active form of caspase 3.
适用于: WB, IHC-Pmore details
与反应: Human
Synthetic peptide corresponding to Human Caspase-3 aa 167-175. Corresponding to the cleavage site of human caspase 3.
Database link: P42574
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: 1% BSA
IgG fraction
Purified immunoglobulin fraction of rabbit antiserum against Caspase-3 containing sodium azide as a preservative.
多克隆
IgG
Abpromise™承诺保证使用ab4051于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 31 kDa. | |
| IHC-P | (6) | Use a concentration of 0.002 mg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. Read More |
Entrez Gene: 836 Human
Omim: 600636 Human
SwissProt: P42574 Human
Unigene: 141125 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (ab4051)This image is courtesy of an anonymous Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of formaldehyde-fixed human brain cortex tissue staining with ab4051 at 1/200 dilution. Secondary antibody was HRP Agilent anti-mouse/rabbit EnVision Dual Link. Samples were incubated with the primary antibody with Agilent antibody diluent for 14 hours at 4°C. Blocking was done using 3% serum for 1 hour at 22°C. Heat mediated antigen retrieval with pH = 6 citrate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (ab4051)
ab4051 staining Caspase-3 in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.
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Anti-Caspase-3 antibody (ab4051) + Human Lung Cell Extract
Predicted band size: 31 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (ab4051)Image courtesy of an anonymous AbReview
ab4051 staining Caspase 3 in Pig liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in milk) for 20 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (ab4051)Image courtesy of an anonymous AbReview
ab4051 staining Caspase 3 in Cow Ovary tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 15 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/75 in PBS + BSA) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
