Anti-Aggrecan antibody [6-B-4]

• 产品名称

  Anti-Aggrecan抗体[6-B-4]
  参阅全部 Aggrecan 一抗

• 描述

  小鼠单克隆抗体[6-B-4] to Aggrecan

• 宿主

  Mouse

• 特异性

  This antibody detects recombinant human Aggrecan by Western Blotting. We have been unable to detect endogenous protein in WB with this antibody. However, there are many citations indicating this antibody is able to detect Aggrecan in endogenous sample lysates.

• 经测试应用

  适用于: WB, IHC-Pmore details

• 种属反应性

  与反应: Human, Recombinant fragment
  

• 免疫原

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• WB: Recombinant human Aggrecan protein. C-terminal 10 His tagged Human Aggrecan recombinant protein (aa 20 to 675). IHC-P: Human cartilage sections.

• 常规说明

  This product has switched from a hybridoma to recombinant production method on 08th October 2020.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.01% Sodium azide
  Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59.04% PBS

• 浓度

• 批次浓度范围 10 µg 浓度为 0.967 - 1.192 mg/ml

• 批次浓度范围 100 µg 浓度为 0.967 - 1.192 mg/ml

• 纯度

  Protein A purified

• Primary antibody说明

  This antibody detects aggrecan metabolites (intact or matrix protease-catabolised) in human synovial fluid samples.

• 克隆

  单克隆

• 克隆编号

  6-B-4

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab3778于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 176 Human

• Omim: 155760 Human

• SwissProt: P16112 Human

• 别名

• AGCAN antibody

• Aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122) antibody

• Aggrecan 1 antibody

• Aggrecan core protein antibody

• Aggrecan proteoglycan antibody

• Aggrecan structural proteoglycan of cartilage antibody

• Aggrecan1 antibody

• ATEGQV antibody

• Cartilage specific proteoglycan core protein antibody

• Chondroitin sulfate proteoglycan 1 antibody

• Chondroitin sulfate proteoglycan 1 large aggregating proteoglycan antigen identified by monoclonal antibody A0122 antibody

• Chondroitin sulfate proteoglycan core protein 1 antibody

• CSPG 1 antibody

• CSPG1 antibody

• CSPGCP antibody

• JSCATE antibody

• Large aggregating proteoglycan antibody

• mcspg antibody

• mgsk16 antibody

• MSK 16 antibody

• MSK16 antibody

• SEDK antibody

• ACAN antibody

• AGC 1 antibody

• AGC1 antibody

• 

  Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778)

  Anti-Aggrecan antibody [6-B-4] (ab3778) at 1/1000 dilution + C-terminal 10 His tagged Human Aggrecan recombinant protein (aa 20 to 675) 15ng
  
  Secondary
  Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
  
  Predicted band size: 250 kDa
  
  
  
  

  Buffer and Concentration: 2% BSA/TBST

  Exposure time: 180 seconds

• 

  Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778)

  ab3778 was shown to react with recombinant human Aggrecan protein in western blot. 

  Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab3778 at 1µg/ml overnight at 4°C. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye®800CW) preabsorbed (ab216772) at a 1/20000 dilution for 1 hour at RT.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)

  Secreted staining of cartilage cells.

  Staining performed on a Leica BOND RXTM system using the standard protocol F. The section was pre-treated using enzymatic retrieval, for 15mins. The section was then incubated with ab3778, 0.05µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)

  No staining in negative control tissue (human normal liver).

  Staining performed on a Leica BOND RXTM system using the standard protocol F. The section was pre-treated using enzymatic retrieval, for 15mins. The section was then incubated with ab3778, 0.05µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• 

  Anti-Aggrecan antibody [6-B-4] (ab3778)