Anti-HMGA1a / HMGA1b抗体
兔多克隆抗体to HMGA1a / HMGA1b
Rabbit
This antibody detects, by Western blot on human GFP fusion proteins in cell lysate, both HMGA1a and the splice variant HMGA1b, but does not detect HMGA2 (see review and Western blot picture). The antibody has not yet been successfully used to detect endogenous protein.
适用于: WB, IHC-P, ChIP, ICC/IFmore details
与反应: Mouse, Human, Zebrafish
预测可用于: Rat, Chicken, Neurospora crassa, Chinese hamster
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
This antibody gave a positive result in human placenta FFPE tissue sections
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab4078于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11.5 kDa). |
| IHC-P | Use at an assay dependent concentration. | |
| ChIP | (1) | Use at an assay dependent concentration. See Abreview submitted on 15 December 2009. |
| ICC/IF | 1/250 - 1/500. Although this antibody gives a specific signal in Immunofluoresence, we have had suggestions that strong background signals are seen. See review by Robert Hock and IF picture below. |
Entrez Gene: 3159 Human
Entrez Gene: 111241 Mouse
Entrez Gene: 15361 Mouse
Omim: 600701 Human
SwissProt: Q9QXP3 Chinese hamster
SwissProt: P17096 Human
SwissProt: P17095 Mouse
Unigene: 518805 Human
Unigene: 703764 Human
Unigene: 426212 Mouse
Unigene: 440289 Mouse
Unigene: 4438 Mouse
Unigene: 469945 Mouse
Unigene: 83614 Rat
High mobility group AT hook 1 antibody
High mobility group AT-hook protein 1 antibody
High mobility group protein A1 antibody
High mobility group protein HMG-I/HMG-Y antibody
High mobility group protein R antibody
HMG-I(Y) antibody
HMGA 1a antibody
HMGA 1b antibody
HMGA1 antibody
HMGA1_HUMAN antibody
HMGI antibody
HMGI/Y antibody
HMGR antibody
HMGY antibody
Western blot - Anti-HMGA1a / HMGA1b antibody (ab4078)This image is courtesy of Monika Harrer
Lanes 1-4 : Anti-HMGA1a / HMGA1b antibody (ab4078) at 1/1000 dilution
Lanes 5-8 : Anti-GFP
Lane 1 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1a GFP fusion proteins.
Lanes 2 & 6 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1b-GFP fusion proteins
Lanes 3 & 7 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA2-GFP fusion proteins
Lane 4 : hole cell lysate from HepG2 cells transfected with plasmids coding for NLS-GFP fusion proteins
Lane 5 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1a-GFP fusion proteins
Lane 8 : Whole cell lysate from HepG2 cells transfected with plasmids coding for NLS-GFP fusion proteins
Secondary
All lanes : Anti-rabbit HRP at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 11.5 kDa
Western blot using ab4078.
Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1a-, hHMGA1b-, hHMGA2- or NLS- (nuclear localisation signal) GFP fusion proteins.
ab4078 was diluted 1/1000 in 5% milk/TBS and incubated for 1 hr at room temperature. Secondary HRP anti-rabbit antibody was diluted 1/10000 in 5% milk/TBS and incubated for 1 hr at room temperature.
Lane 1: hHMGA1a-GFP (ab4078)
Lane 2: hHMGA1b-GFP (ab4078)
Lane 3: hHMGA2-GFP (ab4078)
Lane 4: NLS-GFP (ab4078)
Lane 5: hHMGA1a-GFP (anti-GFP)
Lane 6: hHMGA1b-GFP (anti-GFP)
Lane 7: hHMGA2-GFP (anti-GFP)
Lane 8: NLS-GFP (anti-GFP)
The detected size is that expected for the fusion of an 11kD and 26kD protein (GFP).
Immunocytochemistry/ Immunofluorescence - Anti-HMGA1a / HMGA1b antibody (ab4078)This image is courtesy of Robert Hock
Immunofluoresence on MCF-7 cells transfected with human HMGA1a-GFP using ab4078.
A: Hoechst stain
B: Phasecontrast
C: TexasRed HMG-I/HMG-Y (ab4078)
D: HMGA1a-GFP
E: Merge
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1a / HMGA1b antibody (ab4078)
IHC image of ab4078 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4078, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
