Anti-Estrogen Receptor alpha抗体
参阅全部 Estrogen Receptor alpha 一抗
兔多克隆抗体to Estrogen Receptor alpha
Rabbit
适用于: IHC-P, WB, ICC/IFmore details
与反应: Rat, Human, Recombinant fragment
预测可用于: Hamster, Cow, Cat
Synthetic peptide corresponding to Human Estrogen Receptor alpha aa 21-32.
Sequence:
NELEPLNRPQLK
WB: recombinant fragment; IHC: rat prostate tissue; ICC/IF: T47D and 578T breast cancer cells
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3575于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) | Use a concentration of 1 - 5 µg/ml. |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Estrogen Receptor alpha peptide (ab5847). | |
| ICC/IF | 1/1000 - 1/4000. |
Entrez Gene: 101825539 Hamster
Entrez Gene: 2099 Human
Omim: 133430 Human
SwissProt: Q9QZJ5 Hamster
SwissProt: P03372 Human
Unigene: 208124 Human
Unigene: 10595 Rat
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab3575)
ab3575 at a dilution of 5 µg/ml staining ER alpha in rat prostate tissue sections.
Western blot - Anti-Estrogen Receptor alpha antibody (ab3575)
All lanes : Anti-Estrogen Receptor alpha antibody (ab3575) at 1/1000 dilution
Lane 1 : Recombinant full-length human Estrogen Receptor alpha protein, 250 ng
Lane 2 : Recombinant full-length human Estrogen Receptor alpha protein, 125 ng
Lane 3 : Recombinant full-length human Estrogen Receptor alpha protein, 62.5 ng
Lane 4 : Recombinant full-length human Estrogen Receptor alpha protein, 31.25 ng
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/40000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDa
Blocked with 5% milk in TBST for at least 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody (ab3575)
4% paraformaldehyde fixed 0.1% Triton permeabilized T-47D (human ductal breast epithelial tumor cell line) and negative control line Hs 578T cells stained for Estrogen Receptor alpha (green) using ab3575 (1/2000) in ICC/IF. DyLight 488-conjugated goat anti-rabbit IgG at 1/500.
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