Anti-Granzyme K antibody [GM-24C3]

• 产品名称

  Anti-Granzyme K抗体[GM-24C3]
  参阅全部 Granzyme K 一抗

• 描述

  小鼠单克隆抗体[GM-24C3] to Granzyme K

• 宿主

  Mouse

• 特异性

  This antibody recognises Granzyme K transiently expressed on the cell surface of transfected BOSC cells as well as the native protein in peripheral blood mononuclear cells. It does not cross react with Granzyme A. Specificity is routinely tested by flow cytometry on BOSC cells transiently transfected with a Granzyme K expression vector.

• 经测试应用

  适用于: ELISA, Flow Cyt (Intra), Flow Cytmore details

• 种属反应性

  与反应: Recombinant fragment
  

• 免疫原

  Other Immunogen Type corresponding to Human Granzyme K. Genetic immunization with cDNA encoding human Granzyme K
  Database link: P49863

• 阳性对照

• Flow Cyt: Granzyme K transfected BOSC23 cells.

• 常规说明

  
  

  Granzymes are exogenous serine proteases that are stored in the cytotoxic granules of activated T cells and NK cells. Upon target cell contact, the contents of these granules are directionally exocytosed and, with the assistance of perforin, the granzymes enter the cytosol of the target cell. To date, five human granzymes (A, B, H, K,M) have been described at the molecular genetic level. Human granzyme K (GZMK) is a 28 kD aserine protease whose gene is located on chromosome 5q11-12 close to the granzyme A-encoding gene. Like granzyme A, it has a trypsin-like specifity cleaving at the basic residues arginine and lysine. To which extent human granzyme K plays a role in the induction of apoptosis in the target cells remains to be evaluated. However, granzyme K purified from a rat large granular lymphoma cell line (RNK-16)has been shown to induce apoptosis in vitro. High mRNA levels of granzyme K are detected inactivated T cells and NK cells but are absent in normal tissues that do not contain high numbers of these cells. Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.20
  Constituent: PBS

• 浓度

• 100 µg 浓度为 2 mg/ml

• 纯度

  Protein G purified

• Primary antibody说明

  Granzymes are exogenous serine proteases that are stored in the cytotoxic granules of activated T cells and NK cells. Upon target cell contact, the contents of these granules are directionally exocytosed and, with the assistance of perforin, the granzymes enter the cytosol of the target cell. To date, five human granzymes (A, B, H, K,M) have been described at the molecular genetic level. Human granzyme K (GZMK) is a 28 kD aserine protease whose gene is located on chromosome 5q11-12 close to the granzyme A-encoding gene. Like granzyme A, it has a trypsin-like specifity cleaving at the basic residues arginine and lysine. To which extent human granzyme K plays a role in the induction of apoptosis in the target cells remains to be evaluated. However, granzyme K purified from a rat large granular lymphoma cell line (RNK-16)has been shown to induce apoptosis in vitro. High mRNA levels of granzyme K are detected inactivated T cells and NK cells but are absent in normal tissues that do not contain high numbers of these cells. Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.

• 克隆

  单克隆

• 克隆编号

  GM-24C3

• 同种型

  IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab3771于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

别名

• Fragmentin 3 antibody

• Fragmentin-3 antibody

• Fragmentin3 antibody

• GRAK_HUMAN antibody

• Granzyme 3 antibody

• granzyme K (granzyme 3; tryptase II) antibody

• granzyme K (serine protease, granzyme 3; tryptase II) antibody

• Granzyme K antibody

• Granzyme K precursor antibody

• Granzyme-3 antibody

• Granzyme3 antibody

• GranzymeK antibody

• GZMK antibody

• NK TRYP 2 antibody

• NK TRYP2 antibody

• NK tryptase 2 antibody

• NK-TRYP-2 antibody

• NK-tryptase-2 antibody

• NKTRYP2 antibody

• Serine protease granzyme 3 antibody

• TRYP 2 antibody

• TRYP2 antibody

• Tryptase II antibody

• TryptaseII antibody

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  Flow Cytometry - Anti-Granzyme K antibody [GM-24C3] (ab3771)

  Flow cytometric analysis of BOSC23 cells using ab3771. BOSC23 cells were transiently transfected with an expression vector encoding either Granzyme K (red curve) or an irrelevant protein (control transfectant: black curve). Binding of ab3771 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with Granzyme K transfected cells.

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