Anti-Tau (phospho T212)抗体
参阅全部 Tau 一抗
兔多克隆抗体to Tau (phospho T212)
Rabbit
Phosphorylation site-specific antibody selective for the phosphorylated form of human tau containing a phosphate on threonine 212.
The specificity of this antibody refers to P10636-8.
适用于: ICC/IFmore details
与反应: Human
Synthetic peptide corresponding to Human Tau (phospho T212). The antibody was produced against the region of human Tau that contains threonine 212. The sequence is conserved in many species including mouse, rat, rhesus monkey, baboon, cow and goat.
Database link: P10636-8
ICC/IF: SHSY5Y cells.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
浓度
50 µl 浓度为 0.075 mg/ml
Immunogen affinity purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated tau. The final product is generated by affinity chromatography using a tau-derived peptide that is phosphorylated at threonine 212.
多克隆
IgG
Abpromise™承诺保证使用ab4842于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. |
Entrez Gene: 4137 Human
Omim: 157140 Human
SwissProt: P10636 Human
Unigene: 101174 Human
There are 9 isoforms produced by alternative splicing.
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Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho T212) antibody (ab4842)
SHSY5Y cells stained for Tau (green) using ab4842 at 1µg/mL in ICC/IF. Followed by Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody at 1/400 dilution for 45 minutes at room temperature (Panel a). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin. Panel d is a merged image showing nuclear and cytoplasmic localization. Panel e is a no primary antibody control.
