Anti-RING2 / RING1B / RNF2抗体
参阅全部 RING2 / RING1B / RNF2 一抗
山羊多克隆抗体to RING2 / RING1B / RNF2
Goat
Affinity purified Anti-RING1B antibody is directed against human RING1B protein. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, mouse, rat, dog, bovine, frog and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with RING1B from zebrafish, as only a single amino acid residue changes within the immunogen sequence (92% positive by BLAST). Cross reactivity with RING1B homologues from other sources has not been determined.
适用于: WBmore details
与反应: Mouse, Human, Chinese hamster
Synthetic peptide:
EAGPSNKRTKTSDC
, corresponding to amino acids 189-201 of Human RING1B.
RING1B interacts with CP2, a mammalian member of the grainyhead-like family of transcription factors, in vitro and in vivo. The functional consequence of this interaction is repression of CP2-dependent transcription.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1.1 mg/ml
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
Affinity purified Anti-RING1B antibody is directed against human RING1B protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
RING1B interacts with CP2, a mammalian member of the grainyhead-like family of transcription factors, in vitro and in vivo. The functional consequence of this interaction is repression of CP2-dependent transcription.
多克隆
IgG
Abpromise™承诺保证使用ab3832于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | 1/500 - 1/2000. Detects a band of approximately 43 kDa (predicted molecular weight: 37.6 kDa). |
Entrez Gene: 6045 Human
Entrez Gene: 19821 Mouse
Omim: 608985 Human
SwissProt: Q99496 Human
SwissProt: Q9CQJ4 Mouse
Unigene: 591490 Human
Unigene: 482250 Mouse
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Western blot - Anti-RING2 / RING1B / RNF2 antibody - ChIP Grade (ab3832)
Western blot using ab3832 at 1/500. Lysates at 20µg/lane.
Lane 1: 3T3 cell lysate
Lane 2: U937 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Mouse brain lysate
Lane 5: CHO-K1 cell lysate
Secondary ab: rabbit polyclonal to goat IgG ab6741 1/5000
Exposure time: 3 minutes
Western blot using ab3832 at 1/500. Lysates at 20µg/lane.
Lane 2: U937 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Mouse brain lysate
Lane 5: CHO-K1 cell lysate
Secondary ab: rabbit polyclonal to goat IgG ab6741 1/5000 Exposure time: 3 minutes
