Anti-Cytochrome P450 4A/CYP4A11抗体
参阅全部 Cytochrome P450 4A/CYP4A11 一抗
20-HETE synthase antibody
20-hydroxyeicosatetraenoic acid synthase antibody
CP4AB_HUMAN antibody
CYP4A antibody
Cyp4a1 antibody
Cyp4a10 antibody
CYP4A11 antibody
Cyp4a14 antibody
Cyp4a3 antibody
CYP4A7 antibody
CYP4AII antibody
CYPIVA11 antibody
Cytochrome P-450HK-omega antibody
Cytochrome P450 4A1 antibody
Cytochrome P450 4A10 antibody
Cytochrome P450 4A11 antibody
Cytochrome P450 4A14 antibody
Cytochrome P450 4A2 antibody
Cytochrome P450 4A3 antibody
Cytochrome P450 4A7 antibody
Cytochrome P450HL-omega antibody
Fatty acid omega-hydroxylase antibody
Lauric acid omega-hydroxylase antibody
Rabbit
Western blot - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
All lanes : Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573) at 1/1000 dilution
Lane 1 : Rat liver tissue lysate
Lane 2 : H-4-II-E cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 59 kDa
Observed band size: 58 kDawhy is the actual band size different from the predicted?
Chemiluminescent detection
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
ab3573 labelling Cytochrome P450 4A/CYP4A11 (green) in the cytoplasm and membrane of H-4-II-E (rat) cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
ab3573 labelling Cytochrome P450 4A/CYP4A11 in the cytoplasm of Rat liver tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
ab3573 labelling Cytochrome P450 4A/CYP4A11 (green) in the cytoplasm and membrane of HeLa cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
ab3573 labelling Cytochrome P450 4A/CYP4A11 (green) in the cytoplasm and membrane of PC12 cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
ab3573 labelling Cytochrome P450 4A/CYP4A11 in the cytoplasm of Rat kidney tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
ab3573 labelling Cytochrome P450 4A/CYP4A11 in the cytoplasm and membrane of Human liver tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
The immunogen is homologous to rat cytochrome P450 4A2, 4A10, 4A12 and 4A14. Gene synonyms are Cyp4a11, Cyp4a1, Cyp4a8, and Cyp4a3, respectively. The specificity to these specific forms has not been confirmed experimentally. Please note nomenclature for specific forms may differ from mouse to rat.
适用于: IHC-Fr, ICC/IF, IHC-P, WB, IPmore details
与反应: Mouse, Rat, Rabbit, Hamster, Cat, Dog, Human, Pig
Synthetic peptide corresponding to Rat Cytochrome P450 4A/CYP4A11 aa 400-500. The immunogen is homologous to rat cytochrome P450 4A2, A10, A12 and A14.
Database link: P08516
WB: Rat liver tissue lysate, H-4-II-E and HeLa cell lysate
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Whole antiserum
The Cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNA’s which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and w-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). 20-HETE is converted from AA by the 4A family of P450 proteins which includes at least 8 different, though closely related isoforms. 4A1, 4A2, & 4A3 have been cloned from liver, kidney and testis and have been detected in renal, hepatic & brain microvessels.
多克隆
IgG
Abpromise™承诺保证使用ab3573于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | 1/20 - 1/200. | |
| IHC-P | 1/100 - 1/500. | |
| WB | 1/200 - 1/2000. Predicted molecular weight: 59 kDa. | |
| IP | Use at an assay dependent concentration. |
Entrez Gene: 1579 Human
Omim: 601310 Human
SwissProt: Q02928 Human
SwissProt: P14581 Rabbit
Unigene: 1645 Human
Unigene: 726474 Human
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