Anti-Cytochrome P450 3A4/CYP3A4抗体
参阅全部 Cytochrome P450 3A4/CYP3A4 一抗
兔多克隆抗体to Cytochrome P450 3A4/CYP3A4
Rabbit
适用于: WBmore details
与反应: Mouse, Rat, Human
Recombinant full length protein (His-tag) corresponding to Human Cytochrome P450 3A4/CYP3A4. Purified, His-tagged, full length human P450 3A4 fusion protein.
WB: Rat and mouse liver lysate.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
Ammonium Sulphate Precipitation
多克隆
IgG
Abpromise™承诺保证使用ab3572于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa). A 46kDa band is detected in rat liver extracts. |
Entrez Gene: 1576 Human
Omim: 124010 Human
SwissProt: P08684 Human
Unigene: 728751 Human
CP33 antibody
CP34 antibody
CP3A4_HUMAN antibody
CYP3 antibody
CYP3A antibody
CYP3A3 antibody
CYP3A4 antibody
CYPIIIA3 antibody
CYPIIIA4 antibody
Cytochrome P450 3A3 antibody
Cytochrome P450 3A4 antibody
Cytochrome P450 family 3 subfamily A polypeptide 4 antibody
Cytochrome P450 HLp antibody
Cytochrome P450 NF-25 antibody
Cytochrome P450 subfamily IIIA polypeptide 4 antibody
cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3 antibody
cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4 antibody
Cytochrome P450-PCN1 antibody
Glucocorticoid inducible P450 antibody
HLP antibody
MGC126680 antibody
NF 25 antibody
NF25 antibody
Nifedipine oxidase antibody
P450 III steroid inducible antibody
P450 PCN1 antibody
P450, family III antibody
P450C3 antibody
P450PCN1 antibody
Quinine 3 monooxygenase antibody
Quinine 3-monooxygenase antibody
Taurochenodeoxycholate 6 alpha hydroxylase antibody
Taurochenodeoxycholate 6-alpha-hydroxylase antibody
1,8-cineole 2-exo-monooxygenase antibody
Albendazole monooxygenase antibody
Albendazole sulfoxidase antibody
Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)
All lanes : Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572) at 1/2000 dilution
Lane 1 : Mouse liver tissue extract
Lane 2 : Rat liver tissue extract
Lane 3 : Mouse stomach tissue extract
Lane 4 : Rat stomach tissue extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.
Electrophoresed on 4-12% BisTris gel transferred onto a nitrocellulose membrane
Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)
All lanes : Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572) at 1/2000 dilution
Lane 1 : Mouse liver with Skimmed milk
Lane 2 : Rat liver with Skimmed milk
Lysates/proteins at 30 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : HRP conjugated Goat anti-Rabbit IgG (H+L) at 1/5000 dilution
Predicted band size: 57 kDa
Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)
Western blot of Cytochrome P450 3A4/CYP3A4 on rat liver extract using ab3572.
Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)
ab3572 at a dilution of 1/2000 staining Cytochrome P450 3A4/CYP3A4 in rat liver extract by Western blot.
