Annexin V-FITC Apoptosis Staining / Detection Kit

• 产品名称

  Annexin V-FITC Apoptosis Staining / Detection试剂盒
  参阅全部 Annexin V/ANXA5 试剂盒

• 样品类型

  Adherent cells, Suspension cells

• 检测类型

  Direct

• 检测时间

  0h 10m

• 产品概述

  Annexin V-FITC Apoptosis Staining / Detection Kit ab14085 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.

  
  

  The kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining.

  
  

  The Annexin V-FITC reagent contained in the kit is also available as Annexin V-FITC reagent ab14082.

• 说明

  This product is manufactured by BioVision, an Abcam company and was previously called K101 Annexin V-FITC Apoptosis Kit. K101-100 is the same size as the 100 test size of ab14085.

  Soon after initiating apoptosis, cells translocate membrane phosphatidylserine molecules from the inner face of the plasma membrane to the cell surface. Phosphatidylserine on the cell surface is detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for phosphatidylserine.

  For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.

  
  

  The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.

• 平台

  Flow cytometer, Fluorescence microscope

• 存放说明

  Store at +4°C. Please refer to protocols.

• 组件	100 tests
  Annexin V-FITC II	1 x 500µl
  Binding Buffer II	1 x 50ml
  Propidium Iodide II	1 x 500µl

别名

• Anchorin CII

• Annexin 5

• Annexin A5

• Annexin V

• Annexin-5

• Annexin5

• AnnexinA5

• AnnexinV

• ANX 5

• ANX A5

• ANX5

• ANXA5

• ANXA5_HUMAN

• Calphobindin I

• CBP-I

• Endonexin II

• ENX 2

• ENX2

• Lipocortin V

• PAP I

• PAP-I

• Placental anticoagulant protein 4

• Placental anticoagulant protein I

• PLACENTAL PROTEIN 4

• PP 4

• Pp4

• RPRGL3

• Thromboplastin inhibitor

• VAC-alpha

• Vascular anticoagulant-alpha

• 

  Apoptosis in Mouse Cortical Collecting Duct CellsImage courtesy of an anonymous abreview

  Ab14085 was used to determine minor levels of apoptosis (using both the Annexin V-FITC and PI) in mouse cortical collecting duct cellss (mCCDs). mCCD cells were incubated with serum free medium for 48h. The green label on the plasma membrane (Annexin V-FITC) and the absence of nuclear red (PI) staining indicates apoptosis rather than necrosis. Fluorescent microsocpy ws used to analyse the cells.

• 

  Flow cytometery analysis of treated HeLa cells for 48 hoursAlpay et al., PLos One, 9(19), Fig 5B. Doi:10.1371/journal.pone.0105526 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  HeLa cells were harvested with trypsinization together with floating non-viable cells.  Cells were washed with PBS and  suspended in sodium citrate buffer 20 minutes prior to analysis. HeLa cells were treated with Mitoxantrone (MX) and MX +Imatinib for 48 hours. The samples were then stained with Annexin V-FITC Apoptosis Staining/Detection kit (ab14085). A FACSCalibur flow cytometer was used for cell cycle analysis.

   This is a modified version of the original image

• 

  Analysis of apoptosis in prostate cancer cells following treatment with CTA095Guo W et al., PLoS One, 8(8). Fig7b, doi: 10.1371/journal.pone.0070910 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  PC3 cells were seeded at 106 cells/ml and incubated overnight and then treated with CTA095 at various concentrations for 24hours.  Apoptosis was then analyzed using Annexin-V FITC apoptosis detection kit (ab14085).

  This is a modified version of the original image

• 

  Functional Studies - Annexin V-FITC Apoptosis Detection Kit (ab14085)

  Annexin V-FITC/ PI staining of AG06173 primary fibroblasts.
  105 cells were used for analysis. Resuspended cells were incubated with Annexin V-FITC for 15 min in the dark. Propidium iodide (ab14083) was used as a counterstain to discriminate necrotic/ dead cells from apoptotic cells. Left: negative control - AG6173 untreated cells. Right: positive control - AG6173 cells irradiated at 10 Gy.
  Image courtesy of S. Khoronenkova PhD, Gray Institute for Radiation Oncology and Biology, University of Oxford, Oxford, UK.