Anti-Substance P antibody [SP-DE4-21]

• 产品名称

  Anti-Substance P抗体[SP-DE4-21]
  参阅全部 Substance P 一抗

• 描述

  小鼠单克隆抗体[SP-DE4-21] to Substance P

• 宿主

  Mouse

• 特异性

  The antibody reacts specifically with human Substance P. No crossreactivity with related tachykinins (human Neurokinin A, Neurokinin B, and Kassinin).

• 经测试应用

  适用于: ELISA, IHC-P, ICC/IFmore details

• 种属反应性

  与反应: Mouse, Rat, Guinea pig, Human
  预测可用于: Horse, Chicken, Hamster, Cow, Shark, Common marmoset

• 免疫原

  Synthetic peptide corresponding to Human Substance P aa 58-68.
  Sequence:

  RPKPQQFFGLM

  
  Database link: P20366

  Run BLAST with Run BLAST with 

• 阳性对照

• Guinea pig bone marrow. The antibody binds to the substantia gelatinosa.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.40
  Constituent: 100% PBS

• 浓度

• 50 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  SP-DE4-21

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab14184于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• SwissProt: Q60541 Hamster

• SwissProt: P67933 Horse

• SwissProt: P20366 Human

• SwissProt: P41539 Mouse

• SwissProt: P06767 Rat

• Unigene: 2563 Human

• Unigene: 1440 Mouse

• Unigene: 410823 Mouse

• Unigene: 1920 Rat

• Entrez Gene: 281512 Cow

• Entrez Gene: 6863 Human

• Entrez Gene: 21333 Mouse

• Entrez Gene: 24806 Rat

• Omim: 162320 Human

• SwissProt: P19850 Chicken

• SwissProt: P01289 Cow

• SwissProt: P67932 Guinea pig

• 别名

  hide

• neurokinin 2 antibody

• neurokinin A antibody

• neurokinin alpha antibody

• Neuromedin L antibody

• neuropeptide gamma antibody

• neuropeptide K antibody

• NK2 antibody

• NKA antibody

• NKNA antibody

• NPK antibody

• PPT antibody

• preprotachykinin antibody

• protachykinin antibody

• protachykinin-1 antibody

• Substance K antibody

• SubstanceP antibody

• TAC1 antibody

• TAC2 antibody

• TAC2, formerly antibody

• Tachykinin 1 antibody

• tachykinin 2 antibody

• tachykinin 2, formerly antibody

• Tachykinin precursor 1 antibody

• tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2, neuromedin L, neurokinin alpha, neuropeptide K, neuropeptide gamma) antibody

• Tachykinin1 antibody

• TKN1_HUMAN antibody

• C-terminal-flanking peptide antibody

• Hs.2563 antibody

• neurokinin 1 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)This image is courtesy of an Abreview submitted by Carl Hobbs

  ab14184 staining Substance P in mouse spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide) for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (ab14184)

  ICC/IF image of ab14184 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14184, 1ug/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)Image courtesy of an anonymous Abreview.

  ab14184 staining Substance P in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed. Samples were then incubated with ab14184 at a 1/100 dilution for 1 hour. The secondary used was an undiluted HRP conjugated goat anti-mouse polyclonal.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)

  Substance P staining at 1:1000 of the substancia gelatinosa (dorsal horn) of the guinea pig spinal cord using an HRP-conjugated secondary antibody and DAB.