Anti-PAX8抗体
参阅全部 PAX8 一抗
山羊多克隆抗体to PAX8
Goat
This antibody is expected to recognise all five human isoforms (as represented by NP_003457; NP_039245; NP_039246; NP_039247; NP_054698). This antibody detects a strong band at approx 40kDa in Human Liver lysates. The identity of this band is unclear given the existence of 5 isoforms of PAX8 between 450 aa and 287 aa (54-34 kD). It has shown to be specific for PAX8 in experiments with nuclear extracts of PC Cl3 thyroid cells cultured for 7 days without stimulation from serum and thyrotropin (starved cells) and then stimulated with thyrotropin (TSH) for 24 h (TSH stimulated cells). A band representing PAX8 is seen in the stimulated cells at 60kD (although other non-specific background bands are also seen). We have no confirmation of which isoform this represents.
适用于: IHC-P, WBmore details
与反应: Human
预测可用于: Mouse, Rat, Dog
Synthetic peptide corresponding to Human PAX8 aa 1-100 (N terminal).
Database link: Q06710
IHC-P: Human thyroid tissue WB: Human kidney tissue lysate, HeLa cell lysate and Human thyroid cells
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab13611于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 2 - 4 µg/ml. | |
| WB | (1) | Use a concentration of 1.5 - 4.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 48 kDa). A 1 hour primary incubation is recommended for this product. |
Entrez Gene: 7849 Human
Entrez Gene: 18510 Mouse
Omim: 167415 Human
SwissProt: Q06710 Human
SwissProt: Q00288 Mouse
Unigene: 469728 Human
Unigene: 2533 Mouse
Unigene: 10392 Rat
OTTHUMP00000158659 antibody
OTTHUMP00000158660 antibody
OTTHUMP00000203723 antibody
OTTHUMP00000203724 antibody
Paired box 8 antibody
Paired box gene 8 antibody
paired box homeotic gene 8 antibody
Paired box protein Pax 8 antibody
Paired box protein Pax-8 antibody
Paired domain gene 8 antibody
PAX 8 antibody
PAX8 antibody
PAX8_HUMAN antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab13611)
ab13611 (2µg/ml) staining of paraffin embedded Human Human Thyroid Gland following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining shows nuclear stains in the epithelial cells around the follicles.
Western blot - Anti-PAX8 antibody (ab13611)
All lanes : Anti-PAX8 antibody (ab13611) at 1 µg/ml
Lane 1 : Human Kidney tissue lysate (35µg protein in RIPA buffer).
Lane 2 : HeLa cell lysate (35µg protein in RIPA buffer).
Predicted band size: 48 kDa
Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab13611)
ab13611 at 3µg/ml staining PAX8 in human thyroid gland sections by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by microwave in Tris/EDTA buffer pH 9.0. The HRP-staining procedure was used for detection.
Western blot - Anti-PAX8 antibody (ab13611)
Nuclear extract of PC Cl3 thyroid cells (15 µg) cultured during 7 days without stimulation from serum and thyrotropin (starved cells) and then stimulated with thyrotropin (TSH) 0.5 mUI/ml during 24 h (TSH stimulated cells).
PAX8 is seen to be correctly regulated in panel A. Panel B shows an extended view of the same gel with other visible background bands.
The indicated PAX8 band is at 60 kD. (In some gels PAX8 runs at 55-58kD).
This image was kindly supplied as part of the review submitted by Dr Pilar Santisteban.
Nuclear extract of PC Cl3 thyroid cells (15 µg) cultured during 7 days without stimulation from serum and thyrotropin (starved cells) and then stimulated with thyrotropin (TSH) 0.5 mUI/ml during 24 h (TSH stimulated cells). PAX8 is seen to be correctly regulated in panel A. Panel B shows an extended view of the same gel with other visible background bands. The indicated PAX8 band is at
